Cambio - Excellence in Molecular Biology

CRISPR-Cas 9

CRISPR-Cas 9: Reagents for CRISPR-Cas9

Reagents and kits used in CRISPR/Cas 9

AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
ASB71110AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit10 Reactions £356.00 Quantity Add to Order

AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.

BioSearch Tech (Lucigen/Epicentre)

  • Produce micrograms of biotin-RNA or biotin-aRNA
  • Save time with 30 minute high-yield protocol, or extend the incubation to increase yield even further

Applications

  • Production of nonradioactive RNA probes for any purpose, including microarray (transcription profiling) studies, in situ hybridization, and blotting experiments.

The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.

Features

  • Exceptionally high yields. The kit uses Epicentre's AmpliScribe T7-Flash rapid, high-yield in vitro transcription technology to generate the highest possible yield of biotin-RNA.
  • Optimized. In vitro transcription reaction conditions maximize yield of uniformly labeled biotin-RNA for high signal intensity.
  • Convenient. Rnase Inhibitor included.
  • Convenient. Rnase Inhibitor included.
 

Incubation Time (minutes)

30

60 (1 hour)

120 (2 hours)

480 (4 hours)

Template DNA

50 ng

5 - 10 µg

15 - 25 µg

30 - 40 µg

60 - 70 µg

100 ng

15 - 20 µg

35 - 45 µg

70 - 80 µg

130 - 140 µg

500 ng

60 - 70 µg

140 - 150 µg

> 180 µg

> 180 µg

1.0 µg

125 - 135 µg

> 180 µg

> 180 µg

> 180 µg

Table 1. Yield of Biotin-RNA (in mg) from varying amounts of control template DNA from a standard 37°C, 20 ml AmpliScribe T7-Flash Biotin-RNA Transcription Kit reaction over time. Results may vary depending on the template used.

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigens product page, where the latest protocol is available.

Please note this will open a new page or window on your computer.

 

AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit Protocol

(catalogue number ASB71110)

 Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Production of nonradioactive RNA probes for any purpose, including microarray (transcription profiling) studies, in situ hybridization, and blotting experiments.

 

Benefits

  • The kit uses EPICENTRE's AmpliScribe T7-Flash rapid, high-yield in vitro transcription technology to generate the highest possible yield of biotin-RNA.
  • In vitro transcription reaction conditions are optimized to yield optimal levels of uniformly labeled biotin-RNA for high signal intensity.
  • RNase Inhibitor included.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200