Enzymes for Molecular Biology
The range of applications for the enzymes in this section is extensive. Polymerases are useful for labeled DNA probes by nick translation, second-strand cDNA synthesis, strand-displacement amplification and PR and multiplex PCR amplification. Ligases can be applied to ligation of blunt or cohesive-ended DNA fragments, ligation amplification (LCR), repeat Expansion Detection (RED) and molecular cloning of single stranded and double stranded DNA. Other molecular cloning enzymes listed are useful for DNA repair research and study of UV damaged DNA.
EpiScript™ RNase H (-) Reverse Transcriptase
EpiScript™ RNase H- (negative) Reverse Transcriptase, an alternative to Superscript® II, is a recombinant MMLV reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT demonstrates significantly more activity than other MMLV reverse transcriptase enzymes.
CircLigase™ RNA Ligase
CircLigase™ RNA Ligase (formerly Thermostable RNA Ligase*) is an ATP-dependent ligase that catalyzes intra- and intermolecular ligation of single-stranded RNA (ssRNA) molecules having a 5'-phosphate and a 3'-hydroxyl group.
Ampligase® Thermostable DNA Ligase
Ampligase® Thermostable DNA Ligase catalyses NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. Its half-life is 48 hours at 65°C and greater than 1hour at 95°C.
Exonuclease III digests duplex DNA in a 3'Æ5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible results.