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CRISPR-Cas 9

CRISPR-Cas 9: Reagents for CRISPR-Cas9

Reagents and kits used in CRISPR/Cas 9

AmpliScribe™ T7-Flash™ Transcription Kit

The AmpliScribe™ T7-Flash™ Transcription Kits, EPICENTRE's in vitro transcription kit, produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
ASF3257AmpliScribe™ T7-Flash™ Transcription Kit 25 Reactions £249.00 Quantity Add to Order
ASF3507AmpliScribe™ T7-Flash™ Transcription Kit50 Reactions £345.00 Quantity Add to Order

Description

The AmpliScribe™ T7-Flash™ Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours (Figure 1).
AmpliScribe™ T7-Flash™ Transcription reactions utilize high concentrations of NTPs that are inhibitory to conventional in vitro transcription systems. Using 1 µg of a 1.4 kb DNA template, the standard 20 µl AmpliScribe T7-Flash reactions produce 160 µg to 180 µg (8 to 9 mg/ml) of RNA in 30 minutes. Thus, in 30 minutes, AmpliScribe™ T7-Flash™ reactions produce more RNA than competitors' 'high yield' transcription kits can produce in two hours.

 

Double-stranded oligos, linearized plasmids or PCR products containing a phage T7 or T3 promoter are efficiently transcribed in an AmpliScribe™ T7-Flash™ or AmpliScribe™ T3-Flash™ transcription reaction.

 



Figure 1. AmpliScribe T7-Flash transcription reaction is complete in 30 minutes and yields more RNA than other 2-hour high-yield transcription reactions. Figure 1. An AmpliScribe™ T7-Flash™ transcription reaction is complete in 30 minutes and yields more RNA than other 2-hour "high-yield" transcription reactions. Yields shown were obtained using 1 µg of a linear DNA template in a 20-µl reaction, producing a 1.4-kb RNA

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Protocols

Protocols for: AmpliScribe™ T7 & T3-Flash™ Transcription Kits

 

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigens product page, where the latest protocol is available.

 

Please note this will open a new page or window on your computer.

 AmpliScribe™ T7 & T3-Flash™ Transcription Kits Protocol


(catalogue number ASF3057 / ASF3257 / ASF3507 / ASF03725 / ASF03750)

 Please note: all protocols off site are the responsibility of the products supplier

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Notes

  • For high-yield transcription of templates having an SP6 promoter, use the AmpliScribe SP6 High Yield Transcription Kit.
  • To prepare transcripts that are completely resistant to RNase A, use the DuraScribe® T7 or SP6 Transcription Kits.
  • Use AmpliCap™ High Yield Message Maker Kits for efficient production of RNA with an m7G[5´]ppp[5´]G cap structure on the 5´ end.
  • Because of the very high concentration of NTPs used in AmpliScribe reactions, this kit is not recommended for preparing high specific-activity radiolabeled RNA probes.

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Applications & Benefits

Applications

  • Rapid, maximum-yield synthesis of RNA for studies of RNA structure, processing, ribozymes, RNAi, or antisense RNA.

 

Benefits

  • Exceptionally high yields of full-length RNA. Reactions can be scaled up to obtain grams (or more) of RNA.
  • When transcribing 1 µg of 1-kb linearized plasmid DNA template, a standard AmpliScribe T7-Flash reaction is complete in 30 minutes and yields up to 180 µg of RNA.
  • RNase inhibitor included.
  • Can be used for "Eberwine"-type RNA amplification procedures. However, we recommend TargetAmp™ 2-Round aRNA Amplification Kits for amplification of mRNA from laser-capture microdissection or other small samples.

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Related products

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