EpiScript™ RNase H (-) Reverse Transcriptase
EpiScript™ RNase H- (negative) Reverse Transcriptase, an alternative to Superscript® II, is a recombinant MMLV reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT demonstrates significantly more activity than other MMLV reverse transcriptase enzymes.
CircLigase™ RNA Ligase
CircLigase™ RNA Ligase (formerly Thermostable RNA Ligase*) is an ATP-dependent ligase that catalyzes intra- and intermolecular ligation of single-stranded RNA (ssRNA) molecules having a 5'-phosphate and a 3'-hydroxyl group.
Ampligase® Thermostable DNA Ligase
Ampligase® Thermostable DNA Ligase catalyses NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures. Derived from a thermophilic bacterium, the enzyme is stable and active at much higher temperatures than conventional DNA ligases. Its half-life is 48 hours at 65°C and greater than 1hour at 95°C.
Exonuclease III digests duplex DNA in a 3'Æ5' direction from a blunt end, 5'-overhang or nick, producing stretches of single-stranded DNA. Under defined reaction conditions, DNA degradation by Exonuclease III proceeds at a uniform rate yielding predictable and reproducible results.