The AmpliScribe™ T7-Flash™ Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours (Figure 1).
AmpliScribe™ T7-Flash™ Transcription reactions utilize high concentrations of NTPs that are inhibitory to conventional in vitro transcription systems. Using 1 µg of a 1.4 kb DNA template, the standard 20 µl AmpliScribe T7-Flash reactions produce 160 µg to 180 µg (8 to 9 mg/ml) of RNA in 30 minutes. Thus, in 30 minutes, AmpliScribe™ T7-Flash™ reactions produce more RNA than competitors' 'high yield' transcription kits can produce in two hours.
Double-stranded oligos, linearized plasmids or PCR products containing a phage T7 or T3 promoter are efficiently transcribed in an AmpliScribe™ T7-Flash™ or AmpliScribe™ T3-Flash™ transcription reaction.
||Figure 1. An AmpliScribe™ T7-Flash™ transcription reaction is complete in 30 minutes and yields more RNA than other 2-hour "high-yield" transcription reactions. Yields shown were obtained using 1 µg of a linear DNA template in a 20-µl reaction, producing a 1.4-kb RNA
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