Cambio - Excellence in Molecular Biology

CRISPR-Cas 9

CRISPR-Cas 9: Reagents for CRISPR-Cas9

Reagents and kits used in CRISPR/Cas 9

AmpliScribe™ T7-Flash™ Transcription Kit

The AmpliScribe™ T7-Flash™ Transcription Kits, EPICENTRE's in vitro transcription kit, produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
ASF3257AmpliScribe™ T7-Flash™ Transcription Kit 25 Reactions £193.00£193.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order
ASF3507AmpliScribe™ T7-Flash™ Transcription Kit50 Reactions £280.00£280.00Offer until : 31-Dec-2020Contact Cambio for special pricing on all orders over £2000 View Offer Quantity Add to Order

AmpliScribe™ T7-Flash™ Transcription Kit

The AmpliScribe™ T7-Flash™ Transcription Kits, EPICENTRE's in vitro transcription kit, produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

BioSearch Tech (Lucigen/Epicentre)

The AmpliScribe™ T7-Flash™ Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours (Figure 1).
AmpliScribe™ T7-Flash™ Transcription reactions utilize high concentrations of NTPs that are inhibitory to conventional in vitro transcription systems. Using 1 µg of a 1.4 kb DNA template, the standard 20 µl AmpliScribe T7-Flash reactions produce 160 µg to 180 µg (8 to 9 mg/ml) of RNA in 30 minutes. Thus, in 30 minutes, AmpliScribe™ T7-Flash™ reactions produce more RNA than competitors' 'high yield' transcription kits can produce in two hours.

 

Double-stranded oligos, linearized plasmids or PCR products containing a phage T7 or T3 promoter are efficiently transcribed in an AmpliScribe™ T7-Flash™ or AmpliScribe™ T3-Flash™ transcription reaction.

 



Figure 1. AmpliScribe T7-Flash transcription reaction is complete in 30 minutes and yields more RNA than other 2-hour high-yield transcription reactions. Figure 1. An AmpliScribe™ T7-Flash™ transcription reaction is complete in 30 minutes and yields more RNA than other 2-hour "high-yield" transcription reactions. Yields shown were obtained using 1 µg of a linear DNA template in a 20-µl reaction, producing a 1.4-kb RNA

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

AmpliScribe™ T7-Flash™ Transcription Kit

The AmpliScribe™ T7-Flash™ Transcription Kits, EPICENTRE's in vitro transcription kit, produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: AmpliScribe™ T7 & T3-Flash™ Transcription Kits

 

Due to the constant updating of the protocols by the manufacturer we have provided a direct link to Lucigens product page, where the latest protocol is available.

 

Please note this will open a new page or window on your computer.

 

 AmpliScribe™ T7 & T3-Flash™ Transcription Kits Protocol

(catalogue number ASF3057 / ASF3257 / ASF3507 / ASF03725 / ASF03750)

 Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

AmpliScribe™ T7-Flash™ Transcription Kit

The AmpliScribe™ T7-Flash™ Transcription Kits, EPICENTRE's in vitro transcription kit, produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

BioSearch Tech (Lucigen/Epicentre)

  • For high-yield transcription of templates having an SP6 promoter, use the AmpliScribe SP6 High Yield Transcription Kit.
  • To prepare transcripts that are completely resistant to RNase A, use the DuraScribe® T7 or SP6 Transcription Kits.
  • Use AmpliCap™ High Yield Message Maker Kits for efficient production of RNA with an m7G[5´]ppp[5´]G cap structure on the 5´ end.
  • Because of the very high concentration of NTPs used in AmpliScribe reactions, this kit is not recommended for preparing high specific-activity radiolabeled RNA probes.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

AmpliScribe™ T7-Flash™ Transcription Kit

The AmpliScribe™ T7-Flash™ Transcription Kits, EPICENTRE's in vitro transcription kit, produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Rapid, maximum-yield synthesis of RNA for studies of RNA structure, processing, ribozymes, RNAi, or antisense RNA.

 

Benefits

  • Exceptionally high yields of full-length RNA. Reactions can be scaled up to obtain grams (or more) of RNA.
  • When transcribing 1 µg of 1-kb linearized plasmid DNA template, a standard AmpliScribe T7-Flash reaction is complete in 30 minutes and yields up to 180 µg of RNA.
  • RNase inhibitor included.
  • Can be used for "Eberwine"-type RNA amplification procedures. However, we recommend TargetAmp™ 2-Round aRNA Amplification Kits for amplification of mRNA from laser-capture microdissection or other small samples.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200