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Oligo Synthesis

Oligo Synthesis : CEPs

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5-Me-U-CE Phosphoramidite

5-Me-U-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3050-025-Me-U-CE Phosphoramidite 0.25g £380.00£349.60Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-3050-905-Me-U-CE Phosphoramidite 100µmoles £152.00£139.84Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-3050-955-Me-U-CE Phosphoramidite 50µmoles £76.00£69.92Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

5-Me-U-CE Phosphoramidite

5-Me-U-CE Phosphoramidite

Glen Research

5-Me-U-CE Phosphoramidite

Structure

Catalog Number: 10-3050-xx

Description: 5-Me-U-CE Phosphoramidite

5'-Dimethoxytrityl-5-Methyl-Uridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C46H63N4O9PSi M.W.: 875.08 F.W.: 320.19

Diluent: Anhydrous Acetonitrile
Coupling: 12 minute coupling time recommended
Deprotection: RNA deprotection. See Technical Bulletin for details (www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf). Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

minor rna phosphoramidites (TBDMS PROTECTED)

Inosine and 5-Methyl-Uridine are useful for analyzing RNA structure and activity relationships. 5-Bromo-Uridine and 5-Iodo-Uridine have been used for crystallography studies and cross-linking experiments. 6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, as well as in RNA-protein interactions. The removal of the silyl protecting group without interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride (TBAF) leads to degradation of the thio-nucleotide analogue and should not be used. 2-Aminopurine riboside is useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

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5-Me-U-CE Phosphoramidite

5-Me-U-CE Phosphoramidite

Glen Research

MSDS

Glen Report 19.1: Modified RNA Phosphoramidites Useful In Sirna Research And Biologically Significant 1-Methy-Adenosine

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5-Me-U-CE Phosphoramidite

5-Me-U-CE Phosphoramidite

Glen Research

In Sirna Research And Biologically Significant 1-Methy-Adenosine

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3050-02 0.25grams .25grams 2.86 82 49.2 30.75 22.36 16.4 4.1
10-3050-90 100µmoles .088grams 1 20 12 7.5 5.45 4 1
10-3050-95 50µmoles .044grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3050-02 0.25grams .25grams 4.26 .07 N/A N/A 16.42 N/A
10-3050-90 100µmoles .088grams 1.5 .07 N/A N/A 4.92 N/A
10-3050-95 50µmoles .044grams .75 .07 N/A N/A 1.79 N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3050-02 0.25grams .25grams 4.26 .07 N/A 12.97 12.18

10-3050-90 100µmoles .088grams 1.5 .07 N/A 4.06 3.82

10-3050-95 50µmoles .044grams .75 .07 N/A 1.65 1.55

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200