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Oligo Synthesis

Oligo Synthesis : CEPs

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5-Me-dC Brancher Phosphoramidite

5-Me-dC Brancher Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1018-025-Me-dC Brancher Phosphoramidite 0.25g £404.00£371.68Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1018-905-Me-dC Brancher Phosphoramidite 100µmoles £164.00£150.88Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

5-Me-dC Brancher Phosphoramidite

5-Me-dC Brancher Phosphoramidite

Glen Research

5-Me-dC Brancher Phosphoramidite

Structure

Catalog Number: 10-1018-xx

Description: 5-Me-dC Brancher Phosphoramidite

5'-Dimethoxytrityl-N4-(O-levulinyl-6-oxyhexyl)-5-Methyl-2'-deoxyCytidine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C51H68N5O10P M.W.: 942.10 F.W.: 402.36

Diluent: Anhydrous Acetonitrile
Coupling: dC Brancher reacts in a manner identical to normal phosphoramidites.
Levulinyl Deprotection: The levulinyl protecting group can be selectively removed without cleavage of the oligonucleotide from the CPG by treatment with 0.5 M Hydrazine hydrate in 1:1 pyridine/acetic acid. [Note hydrazine hydrate is a violent poison that is both volatile and readily absorbed through skin] Fit the column with syringes and push the solution back and forth across the column. Let sit for 15 minutes at room temperature. Rinse the column with 1.5 mL of 1:1 pyridine/acetic acid (3x) and then 1.5 mL of ACN (3x). Dry CPG under a stream of argon and proceed with the synthesis of the branching sequence. If a non-branching control is desired, simply deprotect in ammonium hydroxide as required by the nucleobases. Technical Bulletin
Storage: Refrigerated storage, maximum of 2-8°C, dry
Stability in Solution: 24 hours

BRANCHING PHOSPHORAMIDITE

A branching monomer is required to construct comb-like oligonucleotide probes. The developers of the comb system from Chiron Corporation evaluated3 several protecting groups for the branch point and chose levulinyl (LEV), which is specifically removed using a reagent containing hydrazine hydrate, acetic acid and pyridine.

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5-Me-dC Brancher Phosphoramidite

5-Me-dC Brancher Phosphoramidite

Glen Research

5-Me-dC Brancher Phosphoramidite

5-Me-dC Brancher Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1018-02 0.25grams .25grams 2.65 75 45 28.13 20.45 15 3.75
10-1018-90 100µmoles .094grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1018-02 0.25grams .25grams 3.96 .07 72.8 45.5 33.09 4.55
10-1018-90 100µmoles .094grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1018-02 0.25grams .25grams 3.96 .07 74.4 46.5 33.82

10-1018-90 100µmoles .094grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200