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Oligo Synthesis

Oligo Synthesis : CEPs

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5'-Carboxy-Modifier C10

5'-Carboxy-Modifier C10

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1935-025'-Carboxy-Modifier C10 0.25g £212.00£201.40Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1935-905'-Carboxy-Modifier C10 100µmoles £52.00£49.40Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

5'-Carboxy-Modifier C10

5'-Carboxy-Modifier C10

Glen Research

 

Structure

Catalog Number: 10-1935-xx

Description: 5'-Carboxy-Modifier C10

10-Carboxy-decyl-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite,
N-hydroxysuccinimide ester
Formula: C23H40N3O6P M.W.: 485.56 F.W.: varies, -CO2H = 250.23

Diluent: Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: See The Glen Report, Volume 15, Number 1,March 2002, page 11.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: 5'-Carboxy-Modifier C10 is offered for sale under license from TriLink BioTechnologies, Inc. It is intended for research and development purposes only, and may not be used for commercial, clinical, diagnostic or any other use. It is covered under US Patent No. 6,320,041.
Note: NaOH is not compatible with dmf protecting groups.

The disulfide thiol modifier may be used for introducing 3’- or 5’-thiol linkages. Dithiol Phosphoramidite (DTPA) is a disulfide-containing modifier designed to functionalize synthetic DNA or RNA with multiple thiol groups and can be incorporated at any position of the oligonucleotide. Each DTPA addition leads to two thiol groups. This modifier was designed for optimal tethering of oligonucleotides to a gold surface but it can also be used for multiple reactions with maleimides and other thiol-specific derivatives. 5’-Carboxy-Modifier C10 is a unique linker designed to be added at the terminus of an oligonucleotide synthesis. It generates an activated carboxylic acid N-hydroxysuccinimide (NHS) ester suitable for immediate conjugation on the synthesis column with molecules containing a primary amine, resulting in a stable amide linkage. PC Amino-Modifier is a photocleavable C6 amino-modifier, part of our line of photocleavable (PC) modifiers.

5’-AminoOxy-Modifier 11 is based on a tetraethylene glycol linkage for improved solubility and for reducing the potential negative impact on hybridization of the oligo. The oxime formed from the reaction of alkyloxyamines with aldehydes creates a stable covalent bond. In comparison, the imine formed by the conjugation of primary amines with aldehydes is not stable to acidic or basic conditions and requires subsequent reduction with borohydride to form stable amine conjugates. 5’-Maleimide Modifier Phosphoramidite, developed at the University of Barcelona, incorporates a maleimide cycloadduct that is stable to ammonium hydroxide at room temperature. This phosphoramidite can be incorporated into DNA and RNA with both phosphate and phosphorothioate linkages. A retro–Diels-Alder reaction deprotects the maleimide immediately prior to conjugation.

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5'-Carboxy-Modifier C10

5'-Carboxy-Modifier C10

Glen Research

MSDS

Glen Report 15.1

Glen Report 19.1

Glen Report 23.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Carboxy-Modifier C10

5'-Carboxy-Modifier C10

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1935-02 0.25grams .25grams 5.15 158.33 95 59.38 43.18 31.67 7.92
10-1935-90 100µmoles .049grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1935-02 0.25grams .25grams 7.68 .07 147.2 92 66.91 9.2  
10-1935-90 100µmoles .049grams 1.5 .07 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1935-02 0.25grams .25grams 7.68 .07 148.8 93 67.64    
10-1935-90 100µmoles .049grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200