Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

5'-Aldehyde-Modifier C2 Phosphoramidite

5'-Aldehyde-Modifier C2 Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1933-025'-Aldehyde-Modifier C2 Phosphoramidite 0.25g £260.00£239.20Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1933-905'-Aldehyde-Modifier C2 Phosphoramidite 100µmoles £68.00£62.56Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

5'-Aldehyde-Modifier C2 Phosphoramidite

5'-Aldehyde-Modifier C2 Phosphoramidite

Glen Research

5'-Aldehyde-Modifier C2 Phosphoramidite

ALDEHYDE MODIFICATION

Aldehyde modifiers would be attractive electrophilic substitutions in oligonucleotides since they are able to react with amino groups to form a Schiff’s base, with hydrazino groups to form hydrazones, and with semicarbazides to form semi-carbazones. The Schiff’s base is unstable and must be reduced with sodium borohydride to form a stable linkage but hydrazones and semicarbazides are very stable linkages.

Our collaboration with Epoch Biosciences, a subsidiary of Nanogen, Inc., has allowed us to offer 5'-Aldehyde-Modifier C2 Phosphoramidite. The acetal protecting group is sufficiently hydrophobic for use in RP HPLC and cartridge purification and is readily removed after oligonucleotide synthesis under standard oligonucleotide detritylation conditions with 80% acetic acid / 20% water or 2% aqueous trifluoroacetic acid during cartridge purification.

A formylindole nucleoside analogue has been used to introduce aldehyde groups within an oligonucleotide or at the 5’ terminus. This product has no protecting group on the aldehyde, which means that deprotection of the modified oligonucleotide can be done without changing preferred conditions.

10-1933

Catalog Number: 10-1933-xx

Description: 5'-Aldehyde-Modifier C2 Phosphoramidite

2-[4-(5,5-Diethyl-1,3-dioxan-2-yl)phenoxy]ethan-1-yl-
[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C25H41N2O5P M.W.: 480.58 F.W.: 228.14

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_Aldehyde_Modifier.pdf). Technical Bulletin
Storage: Freezer Storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: This Product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of Epoch Biosciences, Inc. and is made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). Eclipse, Gig Harbor Green, Redmond Red and Yakima Yellow are trademarks of Epoch Biosciences, Inc.

ALDEHYDE MODIFICATION

Aldehyde modifiers would be attractive electrophilic substitutions in oligonucleotides since they are able to react with amino groups to form a Schiff’s base, with hydrazino groups to form hydrazones, and with semicarbazides to form semi-carbazones. The Schiff’s base is unstable and must be reduced with sodium borohydride to form a stable linkage but hydrazones and semicarbazides are very stable linkages.

Our collaboration with Epoch Biosciences, a subsidiary of Nanogen, Inc., has allowed us to offer 5'-Aldehyde-Modifier C2 Phosphoramidite. The acetal protecting group is sufficiently hydrophobic for use in RP HPLC and cartridge purification and is readily removed after oligonucleotide synthesis under standard oligonucleotide detritylation conditions with 80% acetic acid / 20% water or 2% aqueous trifluoroacetic acid during cartridge purification.

A formylindole nucleoside analogue has been used to introduce aldehyde groups within an oligonucleotide or at the 5’ terminus. This product has no protecting group on the aldehyde, which means that deprotection of the modified oligonucleotide can be done without changing preferred conditions.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Aldehyde-Modifier C2 Phosphoramidite

5'-Aldehyde-Modifier C2 Phosphoramidite

Glen Research

MSDS

Glen Report 16.2

Glen Report 20.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Aldehyde-Modifier C2 Phosphoramidite

5'-Aldehyde-Modifier C2 Phosphoramidite

Glen Research

These Products are for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Products are subject to proprietary rights of Epoch Biosciences, Inc. and are made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to the Products and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of the Products. “Commercial use” includes but is not limited to the sale, lease, license or other transfer of the Products or any material derived or produced from them, the sale, lease, license or other grant of rights to use the Products or any material derived or produced from them, or the use of the Products to perform services for a fee for third parties (including contract research).

A simple agreement must be signed before end-users and custom oligo services may purchase these products for use as defined above.
http://www.glenresearch.com/
Reference/Epoch.pdf

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Aldehyde-Modifier C2 Phosphoramidite

5'-Aldehyde-Modifier C2 Phosphoramidite

Glen Research

5'-Aldehyde-Modifier C2 Phosphoramidite

2-[4-(5,5-Diethyl-1,3-dioxan-2-yl)phenoxy]ethan-1-yl-
[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C25H41N2O5P M.W.: 480.58 F.W.: 228.14

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: See technical bulletin. (http://www.glenresearch.com/Technical/TB_Aldehyde_Modifier.pdf) Technical Bulletin
Storage: Freezer Storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: This Product is for research purposes only, and may not be used for commercial, clinical, diagnostic or any other use. The Product is subject to proprietary rights of Epoch Biosciences, Inc. and is made and sold under license from Epoch Biosciences, Inc. There is no implied license for commercial use with respect to this Product and a license must be obtained directly from Epoch Biosciences, Inc. with respect to any proposed commercial use of this Product. "Commercial use" includes but is not limited to the sale, lease, license or other transfer of the Product or any material derived or produced from it, the sale, lease, license or other grant of rights to use the Product or any material derived or produced from it, or the use of the Product to perform services for a fee for third parties (including contract research). Eclipse, Gig Harbor Green, Redmond Red and Yakima Yellow are trademarks of Epoch Biosciences, Inc.
Please see Terms and Conditions of Use
  Material Safety Data Sheet

Literature Highlights

Glen Report 16.2: NEW EPOCH PRODUCTS - 5'-Aldehyde-Modifier C2 phosphoramidite and gig harbor green™ phosphoramidite
Glen Report 20.1: New Products

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1933-02 0.25grams .25grams 5.2 160 96 60 43.64 32 8
10-1933-90 100µmoles .048grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1933-02 0.25grams .25grams 7.76 .067 148.8 93 67.64 9.3  
10-1933-90 100µmoles .048grams 1.5 .067 23.6 14.75 10.73 1.48  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1933-02 0.25grams .25grams 7.76 .067 150.4 94 68.36    
10-1933-90 100µmoles .048grams 1.5 .067 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

5'-Aldehyde-Modifier C2 Phosphoramidite

5'-Aldehyde-Modifier C2 Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1933-02 0.25grams .25grams 5.2 160 96 60 43.64 32 8
10-1933-90 100µmoles .048grams 1 20 12 7.5 5.45 4 1
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1933-02 0.25grams .25grams 7.76 .07 148.8 93 67.64 9.3
10-1933-90 100µmoles .048grams 1.5 .07 23.6 14.75 10.73 1.48
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1933-02 0.25grams .25grams 7.76 .07 150.4 94 68.36

10-1933-90 100µmoles .048grams 1.5 .07 25.2 15.75 11.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200