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Oligo Synthesis

Oligo Synthesis : CEPs

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2'-F-U-CE Phosphoramidite

2'-F-U-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3430-022'-F-U-CE Phosphoramidite 0.25g £40.00£38.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-3430-052'-F-U-CE Phosphoramidite 0.5g £80.00£76.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

2'-F-U-CE Phosphoramidite

2'-F-U-CE Phosphoramidite

Glen Research

2'-F-U-CE Phosphoramidite

Structure

Catalog Number: 10-3430-xx

Description: 2'-F-U-CE Phosphoramidite

5'-Dimethoxytrityl-deoxyUridine, 2'-fluoro-3'-[(2-cyanoethyl)-
(N,N-diisopropyl)]-phosphoramidite
Formula: C39H46FN4O8P M.W.: 748.79 F.W.: 308.16

Diluent: Anhydrous Acetonitrile
Coupling: 3 minute coupling time recommended.
Deprotection: Ammonium Hydroxide for 17 hours at 55°C or 30% Ammonia Hydroxide/40% Methylamine 1:1 (AMA) for 2 hours at Room Temperature. (Note - heating in AMA will lead to some degradation of the 2'-Fluoro nucleotides.)
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours
Please Note: A license may be required from Isis Pharmaceuticals, Inc. to incorporate 2'-fluoro modified nucleosides into oligonucleotides as claimed in US Patent Numbers 5,670,633; 6,005,087; 6,531,584 and foreign equivalents.

2’-F-RNA Monomers

2’-Deoxy-2’-fluoro-nucleosides adopt an RNA-type sugar conformation, presumably due to the high electronegativity of fluorine. Because of this sugar conformation, RNA duplexes (A-form) are generally more thermodynamically stable than DNA duplexes (B-form). As expected, the addition of 2’-F-RNA residues to oligodeoxynucleotides progressively increases the thermal stability of their duplexes with RNA. The stabilization is additive at approximately 2° per residue. This compares favorably with 2’-OMe-RNA at around 1.5° and RNA at 1.1° per residue. In the meantime, base pair specificity remains intact.

2’-F-RNA phosphodiester linkages are not nuclease resistant, although the corresponding phosphorothioate linkages are highly resistant. Researchers usually design antisense oligonucleotides to form duplexes with RNA, which are then substrates for RNase H. Uniformly modified 2’-F-RNA/RNA duplexes are not substrates for RNase H. However, it is straightforward to prepare chimeric 2’-F-RNA/DNA phosphorothioate oligonucleotides which exhibit enhanced binding to the RNA target, are substrates for RNase H, and are highly nuclease resistant.

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2'-F-U-CE Phosphoramidite

2'-F-U-CE Phosphoramidite

Glen Research

MSDS

Glen Report 10.1

Glen Report 17.1

Glen Report 21.1

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

2'-F-U-CE Phosphoramidite

2'-F-U-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3430-02 0.25grams .25grams 3.34 98 58.8 36.75 26.73 19.6 4.9
10-3430-05 0.5grams .5grams 6.68 209.33 125.6 78.5 57.09 41.87 10.47
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3430-02 0.25grams .25grams 4.98 .07 93.2 58.25 42.36 5.83
10-3430-05 0.5grams .5grams 9.97 .07 193 120.63 87.73 12.06
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3430-02 0.25grams .25grams 4.98 .07 94.8 59.25 43.09

10-3430-05 0.5grams .5grams 9.97 .07 194.6 121.63 88.45

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200