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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

N-POM Caged-dT-CE Phosphoramidite

N-POM Caged-dT-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1534-02N-POM Caged-dT-CE Phosphoramidite0.25g £716.00£680.20Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1534-90N-POM Caged-dT-CE Phosphoramidite100 µmole £284.00£269.80Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1534-95N-POM Caged-dT-CE Phosphoramidite50 µmole £148.00£140.60Offer until : 31-Aug-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

N-POM Caged-dT-CE Phosphoramidite

N-POM Caged-dT-CE Phosphoramidite

Glen Research

N-POM Caged-dT-CE Phosphoramidite

Structure

Catalog Number: 10-1534-xx

Description: N-POM Caged-dT-CE Phosphoramidite

5'-Dimethoxytrityl-N3-[[1-(6-nitro-1,3-benzodioxol-5-yl)ethoxy]methyl]-2'-deoxyThymidine,
3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C50H58N5O13P M.W.: 967.99 F.W.: 527.38 (N-POM-dT) FW: 304.20 (dT)

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Storage: Refrigerated storage, maximum of 2-8°C, dry. Light sensitive material.
Stability in Solution: 1-2 days

CAGED NUCLEOSIDES

Glen Research’s interest lies in the preparation of caged oligonucleotides whose function is restored after uncaging by UV light at a wavelength that causes no DNA damage. The Deiters group at North Carolina State University has described NPOM-Caged-dT, where the nucleobase is caged with the photolabile group, 6-nitropiperonyloxymethyl (NPOM), which can be removed using UV light at 365nm. Oligonucleotides containing NPOM-Caged-dT every five or six bases do not hybridize to their complementary strand. Photo-uncaging of the caged oligonucleotide is then easily carried out with UV light at 365 nm for seconds to minutes to restore the activity of the oligonucleotide.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

N-POM Caged-dT-CE Phosphoramidite

N-POM Caged-dT-CE Phosphoramidite

Glen Research

Material Safety Data Sheet

Glen Report 23.2: New Product - NPOM-Caged-dT


If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

N-POM Caged-dT-CE Phosphoramidite

N-POM Caged-dT-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1534-02 0.25grams .25grams 2.58 72.67 43.6 27.25 19.82 14.53 3.63
10-1534-90 100µmoles .097grams 1 20 12 7.5 5.45 4 1
10-1534-95 50µmoles .048grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1534-02 0.25grams .25grams 3.85 .07 70.6 44.13 32.09 4.41
10-1534-90 100µmoles .097grams 1.5 .07 23.6 14.75 10.73 1.48
10-1534-95 50µmoles .048grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1534-02 0.25grams .25grams 3.85 .07 72.2 45.13 32.82

10-1534-90 100µmoles .097grams 1.5 .07 25.2 15.75 11.45

10-1534-95 50µmoles .048grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200