Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Ferrocene-dT-CE Phosphoramidite

Ferrocene-dT-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1576-02Ferrocene-dT-CE Phosphoramidite0.25g £600.00£552.00Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1576-90Ferrocene-dT-CE Phosphoramidite100umole £280.00£257.60Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1576-95Ferrocene-dT-CE Phosphoramidite50umole £140.00£128.80Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Ferrocene-dT-CE Phosphoramidite

Ferrocene-dT-CE Phosphoramidite

Glen Research

10-1576

Catalog Number: 10-1576-xx

Description: Ferrocene-dT-CE Phosphoramidite

5'-dimethoxytrityloxy-5-[N-acetyl ferrocenyl-aminohexyl)-3-acrylimido]-
2'-deoxyUridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C60H73FeN6O10P M.W.: 1125.07 F.W.: 684.45

Diluent: Anhydrous Acetonitrile
Coupling: 3 minute coupling time recommended
Deprotection: Synthesize using acetyl-protected dC (10-1015-xx) and deprotect in 30% Ammonium Hydroxide/40% Methylamine 1:1 (AMA) at 65°C for 10 minutes OR Synthesize using UltraMild monomers and Cap A (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx, Cap Mix A (40-4210-xx/ 40-4212-xx) and deprotect in Ammonium Hydroxide for 2 hours at room temperature.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 1-2 days

FERROCENE LABELLING

With an excellent stability profile, ferrocene has always attracted considerable interest for DNA labelling to generate probes for electrochemical detection. Based on our Amino-Modifier C6-dT structure, Ferrocene-dT is easily added to oligonucleotides with no disruption of regular hybridization behavior. Multiple incorporations into an oligonucleotide probe are also simply achieved. Oligonucleotides are deprotected using standard techniques. Ferrocene oligonucleotides should be stored under Argon and aqueous solutions should be degassed immediately.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Ferrocene-dT-CE Phosphoramidite

Ferrocene-dT-CE Phosphoramidite

Glen Research

Material Safety Data Sheet

http://www.glenresearch.com/GlenReports/GR22-22.html

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Ferrocene-dT-CE Phosphoramidite

Ferrocene-dT-CE Phosphoramidite

Glen Research

  1. A E Navarro, et al., Nucleic Acids Res., 2004, 32, 5310-5319
  2. H Brisset, A E Navarro, N Spinelli, C Chaix, and B Mandrand, Biotechnology Journal, 2006, 1, 95-98

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Ferrocene-dT-CE Phosphoramidite

Ferrocene-dT-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1576-02 0.25grams .25grams 2.22 60.67 36.4 22.75 16.55 12.13 3.03
10-1576-90 100µmoles .113grams 1 20 12 7.5 5.45 4 1
10-1576-95 50µmoles .056grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1576-02 0.25grams .25grams 3.32 .07 60 37.5 27.27 3.75
10-1576-90 100µmoles .113grams 1.5 .07 23.6 14.75 10.73 1.48
10-1576-95 50µmoles .056grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1576-02 0.25grams .25grams 3.32 .07 61.6 38.5 28

10-1576-90 100µmoles .113grams 1.5 .07 25.2 15.75 11.45

10-1576-95 50µmoles .056grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200