Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

PC Biotin Phosphoramidite

PC Biotin Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-4950-02PC Biotin Phosphoramidite 0.25g £540.00£513.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-4950-90PC Biotin Phosphoramidite 100µmoles £224.00£212.80Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-4950-95PC Biotin Phosphoramidite 50µmoles £116.00£110.20Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

PC Biotin Phosphoramidite

PC Biotin Phosphoramidite

Glen Research

PC Biotin Phosphoramidite

Structure

Catalog Number: 10-4950-xx

Description: PC Biotin Phosphoramidite

1-[2-Nitro-5-(6-(N-(4,4'-dimethoxytrityl))-biotinamidocaproamidomethyl)phenyl]-
ethyl-[2-cyanoethyl-(N,N-diisopropyl)]-phosphoramidite
Formula: C55H72N7O9PS M.W.: 1038.25 F.W.: 597.62

Diluent: Anhydrous Acetonitrile
Coupling: 2 minute coupling time recommended
Deprotection: PC-Biotin is slow to detritylate. If the final DMT-group is to be removed on the synthesizer, we recommend a second deblocking step.Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days
Please Note: Glen Research offers PC Biotin, PC Amino-Modifier and PC Spacer products in association with AmberGen, Inc. and Link Technologies, Ltd. For a commercial application license, please contact AmberGen, Inc., 617-975-0680, http://www.ambergen.com/.

PHOTOCLEAVABLE MONOMERS

PC Biotin Phosphoramidite can be used to prepare 5’-biotinylated oligonucleotides suitable for capture by streptavidin in a mode similar to our popular 5’ Biotin Phosphoramidite. Amino- and thiol-modified oligonucleotides have proven to be very useful for the attachment of a variety of haptens and fluorophores, as well as for the tethering of the oligonucleotides to a diversity of beads and surfaces. PC Amino-Modifier Phosphoramidite is used to prepare 5’-amino-modified oligonucleotides suitable for subsequent photocleavage. PC Spacer Phosphoramidite can be used as an intermediary to attach any modification reagent, available as a phosphoramidite, to the terminus of oligonucleotides. After photocleavage, a 5’-phosphate is generated on the DNA, rendering it suitable for further biological transformations, such as gene construction and cloning after ligation.

A versatile photocleavable DNA building block has been described by researchers in Washington University, Missouri and used in phototriggered hybridization.1 This reagent has also been used in the design of multifunctional DNA and RNA conjugates2 for the in vitro selection of new molecules catalyzing biomolecular reactions. Researchers at Bruker Daltonik in Germany have also developed genoSNIP, a method for single-nucleotide polymorphism (SNP) genotyping by MALDI-TOF mass spectrometry.3 This method uses size reduction of primer extension products by incorporation of the photocleavable linker for phototriggering strand breaks near to the 3' end of the extension primer. PC Linker can be incorporated into oligonucleotides at any position by standard automated DNA synthesis methodology. PC Linker Phosphoramidite has the added advantage in that photocleavage results in monophosphate fragments at both the 3'- and 5'-termini of the oligonucleotide fragments.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

PC Biotin Phosphoramidite

PC Biotin Phosphoramidite

Glen Research

Material Safety Data Sheet

?Glen Report 14.1: PC Biotin and Related Photocleavable Modifiers

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

PC Biotin Phosphoramidite

PC Biotin Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-4950-02 0.25grams .25grams 2.41 67 40.2 25.13 18.27 13.4 3.35
10-4950-90 100µmoles .104grams 1 20 12 7.5 5.45 4 1
10-4950-95 50µmoles .052grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-4950-02 0.25grams .25grams 3.59 .07 65.4 40.88 29.73 4.09
10-4950-90 100µmoles .104grams 1.5 .07 23.6 14.75 10.73 1.48
10-4950-95 50µmoles .052grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-4950-02 0.25grams .25grams 3.59 .07 67 41.88 30.45

10-4950-90 100µmoles .104grams 1.5 .07 25.2 15.75 11.45

10-4950-95 50µmoles .052grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200