MC1061 F-Electrocompetent Cells
High efficiency F-control strain for phage display applications.
Competent Cells include Control DNA and Recovery Medium and are packaged as DUOs
(2 transformations per tube). Recovery Medium is also available
separately. The specified transformation efficiencies are with pUC DNA,
unless indicated otherwise.
MC1061 F- Electrocompetent Cells. A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 1010
cfu/µg) electrocompetent cells for general cloning or Phage Display.
Note that as an F- strain, it cannot be used for re-infection by
filamentous phage. This strain not included in the sample kit.
Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.
Table 1. Competent Cells for Phage Display libraries
Cell Lines |
Transformation Efficiency (cfu/µg pUC DNA)
|
Cloning Methylated DNA
|
BAC, Cosmid Cloning
|
Blue/White Screening
|
TG1 Electrocompetent
|
>4 × 1010
|
YES
|
NO
|
YES IPTG induction required
|
SS320 (MC1061 F') Electrocompetent
|
>4× 1010
|
YES
|
NO
|
YES IPTG induction required
|
MC1061 F- Electrocompetent
|
>4× 1010
|
YES
|
NO
|
YES IPTG induction required
|
ER2738 Electrocompetent
|
>2 × 1010
|
YES
|
NO
|
YES IPTG induction required
|
Genotype
- TG1: [F' traD36 proAB lacIqZ ΔM15] supE thi-1 Δ(lac-proAB) Δ(mcrB-hsdSM)5(rK - mK -)
- SS320 (MC1061F'): F'[proAB lacIqZ ΔM15 Tn10 (TetR)] araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK ) mcrA mcrB1
- ER2738: [F'proA B lacIq Δ(lacZ)M15 zzf::Tn10 (tetr)] fhuA2 glnVΔ(lac-proAB) thi-1Δ(hsdS-mcrB)5
- MC1061 F-: F-, araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK ) mcrA mcrB1
SDS
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