We offer two bacterial strains designed for BAC library construction or transformation of large constructs:
BAC-Optimised Replicator™ v2.0 Electrocompetent Cells (> 1 × 1010
cfu/µg pKanR DNA). These cells are required for on-demand, copy number
amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight®
Kits, thereby increasing DNA yields. BAC-Optimised Replicator Cells are
components of Biosearch Technologies Cloning Kits
E. cloni 10G BAC-Optimised Electrocompetent Cells (> 1 × 107 cfu/µg 150 kb BAC DNA; > 1 × 1010
cfu/µg pUC DNA). The first competent cells developed specifically for
large DNA cloning. Manufactured using a novel procedure that maximises
transformation efficiencies with very large inserts, 10G BAC-Optimised
Cells are an excellent choice for BAC library construction and large
construct transformation.
Table 1. E. cloni Competent Cells for BAC or Large Fragment Cloning
E. cloni Cell Lines |
Transformation Efficiency (cfu/µg pUC or pKanR DNA)
|
Cloning Methylated DNA
|
BAC, Cosmid Cloning
|
Blue/White Screening
|
10G BAC-Optimised Electrocompetent
|
>1 × 1010
|
YES
|
YES
|
YES without IPTG induction
|
BAC-Optimised Replicator v2.0 Electrocompetent
|
>1 × 1010
|
YES
|
YES
|
YES IPTG induction required
|