HI-Control 10G Chemically Competent Cells
Competent Cells include Control DNA and Recovery Medium, and are
packaged as SOLOs (1 transformation per tube). Recovery Medium is also
available separately. The specified transformation efficiencies are with
pUC DNA, unless indicated otherwise.
Key features
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- Reduce leaky expression: The LacI repressor protein
binds to the lacO operator, blocking transcription of downstream genes.
In the absence of an induction agent such as lactose sugar or IPTG,
target protein expression from the adjacent promoter is greatly reduced.
- Obtain high protein yields: Both 10G and BL21(DE3)-based strains
express high levels of protein. For expression from a T7 promoter,
select the HI-Control BL21(DE3) strain. Other lac-based promoters like
T5-lac, tac, trc, and lac, can be used in either HI-Control strain.
- Achieve high efficiency transformations: Highly efficient competent
cells give excellent results from small amounts of plasmid DNA
Product information
The HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.
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Tightly Control Protein Expression |
|
|
|
T7 Promoters |
Non-T7 lac promoters (T5-lac, tac, trc, lac) |
Clone with low background target expression |
Transformation efficiencies |
HI-Control BL21(DE3) |
X |
X |
|
≥ 1×107 cfu/µg |
HI-Control 10G |
|
X |
X |
≥ 1×109 cfu/µg |
Genotypes:
HI-Control BL21 (DE3): F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)
HI-Control 10G: mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 ?80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ− tonA Mini-F lacIq1 (GentR)
Please note that the SUMO Protease and SUMO Fusion tag are modified
from the original native proteins. Native or non-modified control
proteins or proteases will not be compatible.
SDS
Manuals and user guides
HI Control
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