The
dPCR Validation Standards contain pure, quantified genomic DNA of the
selected species or cell lines. These products are designed as a tool to
assist Residual DNA dPCR-based analysis.
Recommended Use
Each dPCR Validation Standard can be applied to generate genomic DNA
Standard curves for the specific species or cell lines of interest, by
serial dilution. A standard curve can be used to convert the measured
DNA in copies/µl through dPCR in fg/µl to provide a quantification of
the residual DNA in conformity to regulatory guidelines. In addition,
the dPCR Validation Standards can be used during downstream processes to
verify the residual DNA concentration of the processed sample during
several purification processes.
Content
Each dPCR Validation Standard contains 1 vial containing 1 ng of
lyophilized genomic DNA and 1 vial of Rehydration Buffer. The expiry
date of the unopened package is given on the package label.
Available Species and Ordering Information
Cat. No. 58-0201: E. coli
Cat. No. 58-0211: CHO
Cat. No. 58-0221: HEK-293
Result Evaluation
These products are designed as a tool to assist Residual DNA dPCR-based analysis:
• MiQuant Residual DNA dPCR E. coli
• MiQuant Residual DNA dPCR CHO
• MiQuant Residual DNA dPCR HEK-293
Required Consumables
1.5 ml reaction tubes, DNA- and RNA-free
Required Lab Devices
• Pipettes with corresponding filter tips (10 and 100 μl)
• Microcentrifuge for 1.5 ml reaction tubes
• Vortex
Shelf Life and Storage
The components must be stored at 2 °C to 8 °C until use. Once
rehydrated, the dPCR Validation Standards can be stored at ≤ -18 °C for a
maximum of 30 days. Repeated freeze-thaw cycles must be avoided.