Cambio - Excellence in Molecular Biology

qPCR and qRT-PCR

qPCR and qRT-PCR: qRT-PCR

To enable qRT-PCR we offer TATAA cDNA Synthesis kits which are fully compatible with the TATAA qPCR kits

ValidPrime® - control for gDNA bkgd in RNA preps

ValidPrime® replaces the need to perform no reverse transcriptase (RT(-)) controls to test for the presence of genomic DNA (gDNA) for all samples in your reverse transcriptase quantitative PCR (RT-qPCR) profiling.

Tataa Biocenter

Catalogue No.DescriptionPack SizePriceQty
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  • £
A105P10 ValidPrime® Human – Probe assay1000 rxns POA Quantity Add to Order
A105P10MValidPrime® Human – MGB assay 1000 rxns POA Quantity Add to Order
A105P25 ValidPrime® Human – Probe assay250 rxns POA Quantity Add to Order
A105S10 ValidPrime® Human – SYBR assay1000 rxns POA Quantity Add to Order
A105S25 ValidPrime® Human – SYBR assay250 rxns POA Quantity Add to Order
A106P10 ValidPrime® Mouse – Probe assay 1000 rxns POA Quantity Add to Order
A106P25 ValidPrime® Mouse – Probe assay 250 rxns POA Quantity Add to Order
A106S10 ValidPrime® Mouse – SYBR assay 1000 rxns POA Quantity Add to Order
A106S25 ValidPrime® Mouse – SYBR assay 250 rxns POA Quantity Add to Order
A107P10 ValidPrime® Rat – Probe assay 1000 rxns POA Quantity Add to Order
A107P25 ValidPrime® Rat – Probe assay 250 rxns POA Quantity Add to Order
A107S10 ValidPrime® Rat – SYBR assay1000 rxns POA Quantity Add to Order
A107S25 ValidPrime® Rat – SYBR assay250 rxns POA Quantity Add to Order
A108P10 ValidPrime® Vertebrate without gDNA – Probe assay1000 rxns POA Quantity Add to Order
A108P25 ValidPrime® Vertebrate without gDNA – Probe assay250 rxns POA Quantity Add to Order
A108S10 ValidPrime® Vertebrate without gDNA – SYBR assay 1000 rxns POA Quantity Add to Order
A108S25 ValidPrime® Vertebrate without gDNA – SYBR assay 250 rxns POA Quantity Add to Order

Description

ValidPrime®  - Control for genomic DNA background in RNA preps

Description

ValidPrime® replaces the need to perform no reverse transcriptase (RT(-)) controls to test for the presence of genomic DNA (gDNA) for all samples in your reverse transcriptase quantitative PCR (RT-qPCR) profiling.

In an expression profiling experiment based on m samples and n assays, traditional set up requires m RT(-) reactions plus m x n qPCR controls.

When using ValidPrime® only m n 1 controls are needed.

ValidPrime® will minimize the amount of control reactions and hence your costs as well as your efforts.

Features

  • Offers a cost-efficient alternative to RT(-) controls
  • Accurately validates qPCR assays in terms of their sensitivity to gDNA
  • Allows correction for gDNA-derived signals
  • Reduces the need for DNase treatment

 

Use ValidPrime®

  • as a control for gDNA background in qPCR
  • for normalization of samples to cell copy number
  • as an endogenous control for CNV applications

 

ValidPrime® is an assay to test for the presence of gDNA in test samples and when combined with a gDNA control sample, replaces all RT(-) controls. ValidPrime® is highly optimised and specific to a non-transcribed locus of gDNA that is present in exactly one copy per haploid normal genome. The sequence detected by the ValidPrime® assay has no transcriptional activity and is not present in pure cDNA preparations. Therefore, ValidPrime® measures the number of genomic copies present in a sample.

In an expression profiling experiment the ValidPrime® assay is added to the list of assays and the gDNA control is added to the list of samples. From the combined measurements of the ValidPrime® assay and the gene of interest (GOI) assays on all samples and on the gDNA control, the genomic background contribution to all RT-qPCR measurements can be assessed. ValidPrime® replaces the need to perform RT(-) controls for all reactions and makes RT-qPCR profiling easier and substantially cheaper.

The assay has very high PCR efficiency (E > 90% in tested commercial master mixes) and produces negligible amount of primer dimer products. Limit of detection (LOD) is estimated to 4 copies of DNA (0.01 ng of DNA) and limit of quantification (LOQ) is estimated to 32 copies
of DNA (0.08 ng of DNA).

For ValidPrime® Human, ValidPrime® Mouse, and ValidPrime® Rat, a gDNA standard is provided which can be used to test the sensitivity of qPCR assays for gDNA background. ValidPrime® Vertebrate does not include a gDNA standard, since the standard needs to be specific for the species analysed and arranged by the user.

 

 

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200