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Lab Reagents

Lab Reagents: Absolute Lectins

Absolute Lectins, perfect for Glycoscience Medicago is a primary manufacturer of a wide variety of exceptionally pure lectins purified by affinity chromatography. Lectins are non-enzymatic proteins of non-immunoglobulin origin that bind specifically and reversibly to carbohydrate moieties without altering the covalent structure of the glycosyl ligands. The term ‘lectin’ is derived from the latin word legere, meaning ‘to select’. The specificity of a lectin is usually defined by the monosaccharides or oligosaccharides that are best at inhibiting the agglutination or precipitation caused by the lectin. Lectins are usually of plant origin but do occur in many types of organism; some are glycoproteins and may be soluble or membrane-bound. The biological function of lectins is far from fully explored. Their specificity enables binding to glycoproteins and polysaccharides as well as agglutination of erythrocytes and stimulation of blood lymphocytes. Because of their ability to distinguish glycosyl ligands on human red blood cells, lectins can be used for blood typing.Immobilized lectins can be used in affinity chromatography to purify and isolate glycoproteins, glycolipids, polysaccharides, viruses and cells. Carbohydrate-containing substances bound to the lectin may be eluted with a competitive binding substance. Medicago offers lectins as a freeze-dried powder or as a solution manufactured under aseptic conditions. If you don’t find the lectin you are looking for, we can certainly produce it for you. Furthermore, we offer different custom lectin conjugates on request. •Ultrapure quality •High activity •Wide range •Lyophilized powder •Custom lectins and conjugates

Pisum sativum lectin (PSA)

Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity chromatography. The lectin has four subunits and a molecular weight of 49 kDa. PSA agglutinates human erythrocytes but is not blood group specific . It displays specificity toward a-D-mannose and a-D-glucose (1) and has mitogenic activity similar to Concanavalin A.

Medicago AB

Catalogue No.DescriptionPack SizePriceQty
MC-05-0111-10Pisum sativum lectin (PSA)10 mg £60.00 Quantity Add to Order
MC-05-0111-100Pisum sativum lectin (PSA)100 mg £383.00 Quantity Add to Order
MC-05-0111-25Pisum sativum lectin (PSA)25 mg £128.00 Quantity Add to Order

Pisum sativum lectin (PSA)

Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity chromatography. The lectin has four subunits and a molecular weight of 49 kDa. PSA agglutinates human erythrocytes but is not blood group specific . It displays specificity toward a-D-mannose and a-D-glucose (1) and has mitogenic activity similar to Concanavalin A.

Medicago AB

Product description

Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity chromatography. The lectin has four subunits and a molecular weight of 49 kDa. PSA agglutinates human erythrocytes but is not blood group specific (1). It displays specificity toward α-D-mannose and α-D-glucose (1) and has mitogenic activity similar to Concanavalin A (2).

Medicago’s Pisum sativum lectin is supplied as a white to cream coloured lyophilized powder. The purity of PSA is determined by SDS- electrophoresis, which generates two bands at 17 kDa and 5,7 kDa corresponding to the α- and ß-chains that are formed in solution, below pH 5.8 (4). The lectin is available in vials containing 100 mg, 25 mg or 10 mg powder and the product is to be used for laboratory work only.


Features 

  • Ultrapure quality
  • Carbohydrate binding specificity: α-D-mannose and α-D-glucose
  • Non-specific blood group agglutinin 
  • Mitogenic activity
  • Lyophilized powder


 Directions for use

The lectin may be reconstituted with 2 ml of deionized water before use. Spin the vial gently until full dissolution. Aggregation is thought to occur in the presence of high concentrations of 2-mercaptoethanol.

 

Shipping and storage

The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pisum sativum lectin (PSA)

Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity chromatography. The lectin has four subunits and a molecular weight of 49 kDa. PSA agglutinates human erythrocytes but is not blood group specific . It displays specificity toward a-D-mannose and a-D-glucose (1) and has mitogenic activity similar to Concanavalin A.

Medicago AB

References

(1) Liener I. E., Sharon N., Goldstein I. J., (1986) The Lectins – Properties, Functions and Applications in Biology and Medicine.

(2) Preedy V. R., Watson R. R. (2005). Reviews in food and nutrition toxicity Vol 4.

(3) The Structure of Pea Lectin-D-Glucopyranose Complex at a 1.9 A Resolution Pletnev, V.Z., Ruzheinikov, S.N., Tsygannik, I.N., Mikhailova Yu, I., Duax, W., Ghosh, D., Pangborn, W. (1997) RUSS. J.Bioorganic Chem. 23: 469.

(4) Schwarz F. P., Puri K. D., Rama G. B., Surolia A (1993) Thermodynamics of Monosaccharide Binding to ConcanavalinA, Pea (Pisum sativum)Lectin, and Lentil (Lens culinaris) Lectin. J Biol Chem. Vol. 268, No.11, p 7668-7677

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pisum sativum lectin (PSA)

Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity chromatography. The lectin has four subunits and a molecular weight of 49 kDa. PSA agglutinates human erythrocytes but is not blood group specific . It displays specificity toward a-D-mannose and a-D-glucose (1) and has mitogenic activity similar to Concanavalin A.

Medicago AB

Specifications

Appearance: White to cream coloured lyophilized powder or flocculate                              

Source: Peas

Molecular weight: 49 kDa

Sugar specificity: α-D-Man and α-D-Glu

Activity: 10-100 μg/ml lectin is required to agglutinate neuraminidase treated red blood cells. Agglutinates human erythrocytes; not blood group specific.

Microorganisms: < 100 CFU/g

Protein content: > 85 %, OD280nm (ε 1.6 = 1 mg/ml)

Identity: Phast-gel PAGE gradient 8-25%, two bands at 17 kDa and 5,7 kDa

Shelf life: > Three years when stored at -20°

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Pisum sativum lectin (PSA)

Pisum sativum lectin or agglutinin (PSA) is isolated from peas and purified by affinity chromatography. The lectin has four subunits and a molecular weight of 49 kDa. PSA agglutinates human erythrocytes but is not blood group specific . It displays specificity toward a-D-mannose and a-D-glucose (1) and has mitogenic activity similar to Concanavalin A.

Medicago AB

Applications

  • Agglutination studies
  • Model system of how proteins recognize carbohydrates

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200