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PCR & qPCR

PCR & qPCR: PCR

The FailSafe™ PCR system for templates up to 20kb long, high GC content (up to 85% G+C), & Multiplex PCR

MegaTaq polymerase

For use in polymerase chain reaction

Cambio

Catalogue No.DescriptionPack SizePriceQty
CA-1724-05 MegaTaq Polymerase500U £80.00 Quantity Add to Order

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MegaTaq polymerase

For use in polymerase chain reaction

Cambio

MegaTaq Polymerase

(Data sheet available here)

Description

  • Highly purified, recombinant protein of approximately 94 kDa from Thermus aquaticus
  • An excellent and affordable Taq polymerase that comes with 10X PCR buffers in a ready-to-use format (either with or without electrophoresis tracking dyes) or wth a buffer that lacks MgCl2 that allows researchers to test and/or use their own optimal magnesium ion concentration for primers and template of choice.

Applications

  • Recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 Kb
  • Optimal amplification of DNA at 74°C  with a half-life of 40 minutes at 95°C
  • Taq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium ions
  • Exhibits 5'→3' exonuclease activity
  • Lacks 3'→5' exonuclease activity

Reagents Supplied

10X Taq PCR Buffer A
10X Taq PCR Buffer B
10X Taq PCR Buffer C

10X SuperTaq Buffer (original formulation available on request)

Unit Definition 

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C

10X Reaction Buffer

10X Taq Buffer A (optimization buffer without MgCl2): Buffer allows to optimize MgCl2 concentration
10X Taq Buffer B (general application, up to 10 kb): Buffer contains 15 mM MgCl2 and is optimized for use with 0.2 mM of each dNTP
10X Taq Buffer C (contains coloured dyes): 10X Taq Buffer B  contains two gel tracking dyes together with and a gel loading reagent; thus enabling the direct loading of PCR products onto an agarose gel without any further sample preparation.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, stabilisers.

Quality Control

Assayed to confirm extremely minimal contaminating endonuclease, 3'-exonuclease, and nonspecific single- and double-stranded DNase activities.

Typical preparations are assessed to be of greater than 95% purity, as judged by SDS polyacrylamide gel electrophoresis.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MegaTaq polymerase

For use in polymerase chain reaction

Cambio

Click here for MSDS

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MegaTaq polymerase

For use in polymerase chain reaction

Cambio

References

1. Chien, A., Edgar, D.B. and Trela, J.M. (1976) J. Bacteriol. 127, 1550
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980) Biokhimiya 45, 644

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

MegaTaq polymerase

For use in polymerase chain reaction

Cambio

Applications

  • Recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 Kb
  • Taq DNA Polymerase enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C (1, 2)
  • Taq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5'→3' direction in the presence of magnesium ions
  • Maintains the 5'→3' exonuclease activity
  • Lacks the 3'→5' exonuclease activity

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200