How to run a PCR marathon: FailSafe Technical Tip
Friday, 9 November 2018
How to run a PCR marathon:
The FailSafe™ PCR Selection Kit is suitable for producing long amplicons up to >20kb in length. It may be used for long PCR products in place of the discontinued MasterAmp™ Extra-Long PCR System.
For optimizing production of amplicons in excess of 10 kb simply use (a) FailSafe 2X PreMixes A through I, and (b) 1 uL of FailSafe DNA Polymerase in a 50 uL reaction.
Identify which PreMix provides the best amplification (i.e. a single, strong PCR product of the correct size or expected multiplex results with the least produced primer-dimer). Use the chosen FailSafe 2X PCR PreMix every time that you perform this particular amplification to ensure consistent and reliable results.
The FailSafe DNA Polymerase Mix contains a 3’-5’ proof-reading enzyme that delivers fidelity at least 3-times higher than Taq DNA Polymerase.
See here to learn more about FailSafe...