Isothermal Nucleic Acid Amplification
Designed for rapid, sensitive nucleic acid amplification without conventional thermal cycling, our products support molecular diagnostic development, point-of-care and agricultural testing, and life science research. Through continued innovation, consistent quality, and reliable performance, we help researchers and industry partners accelerate discovery and develop next-generation testing solutions. Bulk quantities, custom formulations, CDMO and OEM services are also available.
Overview of the RPA Amplification Process
Recombinase Polymerase Amplification (RPA) amplifies DNA at a constant temperature (37-42 °C) using a recombinase (e.g. UvsX), primers, a single-stranded DNA binding protein (SSB), and a strand displacing DNA polymerase. T4 UvsX is used in combination with its accessory protein, UvsY.
The recombinase interacts with the primers to form nucleoprotein filament. This complex is able to bind with homologous double-stranded DNA through a strand exchange. After the exchange, a single-stranded binding protein, T4 gp32, stabilizes the displaced strand. Finally, Bsu/Sau polymerase extends the primers, creating a new complete copy of the template, and amplification can continue like Polymerase Chain Reaction (PCR).
Applications and Benefits
- Highly selective and sensitive isothermal amplification technique.
- Alternative to PCR. No Thermocycler needed.
- Speed and sensitivity. Excellent for Rapid Molecular and Agricultural tests. This isothermal reaction employing T4 UvsX DNA Recombinase does not require pretreatment of DNA, nor does it require any strand denaturation, making it suitable for field tests or performing tests with minimal equipment and supplies.
- Successfully implementation with different detection strategies, from end-point lateral flow strips to real-time fluorescent detection
Intact Genomics offers a variety of Recombinase Polymerase Amplification (RPA) optimization enzymes and kits. All enzymes thoroughly tested for activity, purity and concentration. We are continuously discovering and developing novel classes of RPA enzymes in order to speed up the point-of-care (POC) diagnostic process.
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