Catalog Number: 20-2030-xx
(20-2130-xx, 20-2230-xx, 20-2530-xx)
Description: dT-CPG 500
| 5'-Dimethoxytrityl-2'-deoxyThymidine, 3'-succinoyl-long chain alkylamino-CPG 500 |
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F.W.: 304.20 |
Diluent: Not Applicable |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Deprotect using the protocol required by the nucleobases. |
| Storage: Controlled room temperature or lower, dry |
| Stability in Solution: Not Applicable |
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ABI INSTRUMENTS
- 45mL septum-capped vials used on oldest ABI 380, 381 and 391 instruments. 200mL oxidizer and 450mL deblock screw-capped bottles also used on ABI 380, 381 and 391 instruments.
- Small screw-capped vials used on ABI 392 and 394 instruments.
- Larger screw-capped vials used on ABI 392. 394 and 3400 instruments.
- Large bottles used on ABI 3900 instruments.
ABBREVIATIONS
Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCM = Dichloromethane
dmf = dimethylformamidine
I2 = Iodine
lcaa = long chain alkylamino
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran
SEE ALSO
Alternative Solvents p31
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STERLING SUPPORTS
All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.
|
|
ABI INSTRUMENTS
- 45mL septum-capped vials used on oldest ABI 380, 381 and 391 instruments. 200mL oxidizer and 450mL deblock screw-capped bottles also used on ABI 380, 381 and 391 instruments.
- Small screw-capped vials used on ABI 392 and 394 instruments.
- Larger screw-capped vials used on ABI 392. 394 and 3400 instruments.
- Large bottles used on ABI 3900 instruments.
ABBREVIATIONS
Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCM = Dichloromethane
dmf = dimethylformamidine
I2 = Iodine
lcaa = long chain alkylamino
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran
SEE ALSO
Alternative Solvents p31
|
STERLING SUPPORTS
All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.
|
|
ABI INSTRUMENTS
- 45mL septum-capped vials used on oldest ABI 380, 381 and 391 instruments. 200mL oxidizer and 450mL deblock screw-capped bottles also used on ABI 380, 381 and 391 instruments.
- Small screw-capped vials used on ABI 392 and 394 instruments.
- Larger screw-capped vials used on ABI 392. 394 and 3400 instruments.
- Large bottles used on ABI 3900 instruments.
ABBREVIATIONS
Ac2O = Acetic Anhydride
CE = Cyanoethyl
CPG = Controlled Pore Glass
DCM = Dichloromethane
dmf = dimethylformamidine
I2 = Iodine
lcaa = long chain alkylamino
MeIm = 1-Methylimidazole
µm = micromole(s)
nm = nanomole(s)
TCA = Trichloroacetic Acid
THF = Tetrahydrofuran
SEE ALSO
Alternative Solvents p31
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