High Efficiency Chemically & Electro Competent Agrobacterium Cells

Intact Genomics (IG) is the largest and earliest provider of agrobacterium competent cells in the market. We provide the highest quality agrobacterium competent cells to customers and distributors worldwide. We also developed the first high-efficiency chemically agrobacterium competent cells in 2020. Using propriety methods, Intact Genomics has developed and manufactured a variety of Agrobacterium competent cells to support the development of plant transformation and agricultural research.

Methionine and Thymidine Auxotrophic Agrobacteria

Competent Cells

We also provide a variety of highly unique strains. This includes Methionine and Thymidine Auxotrophic Agrobacteria competent cells. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain. Auxotrophic Agrobacteria help to solve this problem. Methionine or Thymidine Auxotrophic Agrobacterium strains include modifications so that they will not grow unless methionine or thymidine is added to Minimal medium. Using the selections of minimal media without Methionine or Thymidine in combination with selective antibiotics, it completely prevents the bacteria from overgrowing plant tissues during plant transformation.

Intact Genomics’ Methionine Agrobacterium strains were originally cloned and provided by Dr. Wayne Parrott’s lab under license from University of Georgia. Thymidine Agrobacterium strains were originally cloned and provided by Dr. Kan Wang’s lab under license from Iowa State University. Knocking out genes to cause auxotrophy does not affect transformation capacity.

Benefits Include

• Enables development of more efficient transformation systems.
• Reduced bacterial overgrowth during co-cultivation.
• Decreased need for antibiotics.
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Scroll down to find our collection of  Methionine and Thymidine Auxotrophic Agrobacteria competent cells.