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Oligo Synthesis

Oligo Synthesis : CEPs

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2,6-Diaminopurine-TOM-CE Phosphoramidite

2,6-Diaminopurine-TOM-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-3085-022,6-Diaminopurine-TOM-CE Phosphoramidite 0.25g £886.00 Quantity Add to Order
10-3085-902,6-Diaminopurine-TOM-CE Phosphoramidite 100µmoles £386.00 Quantity Add to Order
10-3085-952,6-Diaminopurine-TOM-CE Phosphoramidite 50µmoles £193.00 Quantity Add to Order
40-4450-20Diluent optional Anhydrous Acetonitrile/Dichloromethane20ml £0.00 Quantity Add to Order

Description

2,6-Diaminopurine-TOM-CE Phosphoramidite
 

Catalog Number: 10-3085-xx

Description: 2,6-Diaminopurine-TOM-CE Phosphoramidite

5'-Dimethoxytrityl-N6-isobutyryl-N2-methoxyacetyl-2,6-diaminopurine riboside,
2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C57H81N8O11PSi M.W.: 1113.36 F.W.: 344.22


Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) - code :40-4450-20

Please request with your order if required.

Coupling: See Technical Bulletin
Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf). Technical Bulletin
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

MINOR RNA PHOSPHORAMIDITES (TOM PROTECTED)

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. 4-Thio-U, 5-Methyl-Cytidine, 2-Aminopurine riboside and 2-Amino-Adenosine are useful for analyzing RNA structure and activity relationships, for example, in ribozyme studies.

Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.

rSpacer is used to introduce an a basic site to an RNA sequence.

The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Protocols

MSDS

Glen Report

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Applications & Benefits

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-3085-02 0.25grams .25grams 2.25 61.67 37 23.13 16.82 12.33 3.08
10-3085-90 100µmoles .111grams 1 20 12 7.5 5.45 4 1
10-3085-95 50µmoles .056grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-3085-02 0.25grams .25grams 3.35 .07 N/A N/A 12.63 N/A
10-3085-90 100µmoles .111grams 1.5 .07 N/A N/A 4.92 N/A
10-3085-95 50µmoles .056grams .75 .07 N/A N/A 1.79 N/A
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-3085-02 0.25grams .25grams 3.35 .07 N/A 10.03 9.42

10-3085-90 100µmoles .111grams 1.5 .07 N/A 4.06 3.82

10-3085-95 50µmoles .056grams .75 .07 N/A 1.65 1.55

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200