Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Clinical

Isolate and purify DNA from clinical materials, such as buccal swabs, blood, saliva tissues and stool samples.

MasterPure™ DNA Purification Kit for Blood Version II

The MasterPure DNA Purification Kit for blood can be used to isolate exceptionally pure genomic DNA from whole mammalian blood or buffy coat.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
MB711400MasterPure™ DNA Purification Kit for Blood Version II400ml of whole Blood £458.00 Quantity Add to Order

Description

The MasterPure™ DNA Purification Kit for blood version II can be used to isolate exceptionally pure genomic DNA, TNA or for RNA from whole mammalian blood or buffy coat

 Up to 100µg of DNA can be recovered from 600µl of buffy coat in less than 30 minutes using only 1.5-ml microcentrifuge tubes. The purified DNA can be used directly as a template in amplification reactions, for digestion with restriction enzymes, or for Southern blotting. Protocols are provided for genomic DNA purification from whole blood.

Starting MaterialYield
600µl Buffy Coat 87-109µl
200µl Whole Blood 3-9µl
0.6µl 9-27µl
0.8µl 12-36µl
1µl 15-45µl
2µl 30-90µl
3µl 45-120µl
4µl 60-180µl
5µl 75-225µl





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Protocols

Protocols for: MasterPure™ DNA Purification Kit for Blood

MasterPure™ DNA Purification Kit for Blood Protocol

(catalogue number MG71100)

Please note: all protocols off site are the responsibility of the products supplier

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Notes

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Applications & Benefits

Applications

  • Purification of genomic DNA from whole mammalian blood or buffy coat for most molecular biology and applications such as PCR amplification

Benefits

  • Consistent yields of up to 10 µg DNA from 325 µl of whole blood and approximately 100 µg DNA from 600 µl of buffy coat.
  • A260/A280 ratios of 1.8-2.0.
  • Protocol can be completed in less than 30 minutes.
  • No columns to reduce yields.
  • No organic solvents are used.
  • Purified DNA is PCR-ready and archive-quality.
  • Scalable protocol for large or small samples.

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Related products

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