Cambio - Excellence in Molecular Biology

Protein Purification

Protein Purification: Apollo 20 mL Ultrafiltration

Apollo ultrafiltration devices are used for purification, concentration or desalting of proteins and viral gene vector particles. They are available in 7 mL and 20 mL versions, each having 3 different membrane retention ratings: 9 kDa, 20 kDa, and 150 kDa.

APOLLO 20 mL CONCENTRATORS

Ultrafiltration using Apollo® Concentrators (US patents 6,269,957, 6,357,601) combines the highest recovery of regenerated cellulose membrane devices with faster concentration of protein samples and viral gene vectors than possible with recent high-speed devices of comparable retention. Apollo ultrafiltration devices are used for purification, concentration or desalting of proteins and viral gene vector particles. They are available in 7 mL and 20 mL versions, each having 3 different membrane retention ratings: 9 kDa, 20 kDa, and 150 kDa.

Orbital Biosciences

Catalogue No.DescriptionPack SizePriceQty
AP-2000904009 kDa APOLLO 20 mL CONCENTRATORS 1 bag of 8 filters, tubes and caps £68.00 Quantity Add to Order
AP-2000910009 kDa APOLLO 20 mL CONCENTRATORS 1 rack of 25 filters, tubes and caps £210.00 Quantity Add to Order
AP-2000922009 kDa APOLLO 20 mL CONCENTRATORS 2 bags of 125 filters only POA Quantity Add to Order
AP-2000942009 kDa APOLLO 20 mL CONCENTRATORS 1 bag of 100 filters only £690.00 Quantity Add to Order
AP-2002004020 kDa APOLLO 20 mL CONCENTRATOR 1 bag of 8 filters, tubes and caps £68.00 Quantity Add to Order
AP-2002010020 kDa APOLLO 20 mL CONCENTRATOR 1 rack of 25 filters, tubes and caps £210.00 Quantity Add to Order
AP-2002022020 kDa APOLLO 20 mL CONCENTRATOR 2 bags of 125 filters only POA Quantity Add to Order
AP-2002042020 kDa APOLLO 20 mL CONCENTRATOR 1 bag of 100 filters only £690.00 Quantity Add to Order
AP-2015004150 kDa APOLLO 20 mL CONCENTRATOR 1 bag of 8 filters, tubes and caps £68.00 Quantity Add to Order
AP-2015010150 kDa APOLLO 20 mL CONCENTRATOR 1 rack of 25 filters, tubes and caps £210.00 Quantity Add to Order
AP-2015022150 kDa APOLLO 20 mL CONCENTRATOR 2 bags of 125 filters only POA Quantity Add to Order
AP-2015042150 kDa APOLLO 20 mL CONCENTRATOR 1 bag of 100 filters only £690.00 Quantity Add to Order

APOLLO 20 mL CONCENTRATORS

Ultrafiltration using Apollo® Concentrators (US patents 6,269,957, 6,357,601) combines the highest recovery of regenerated cellulose membrane devices with faster concentration of protein samples and viral gene vectors than possible with recent high-speed devices of comparable retention. Apollo ultrafiltration devices are used for purification, concentration or desalting of proteins and viral gene vector particles. They are available in 7 mL and 20 mL versions, each having 3 different membrane retention ratings: 9 kDa, 20 kDa, and 150 kDa.

Orbital Biosciences

Apollo 20 mL  High-Performance Concentrators 

with Accelerated Conical Focusing

 

Use these centrifugal ultrafiltration devices with combined simplicity, speed, capacity and recovery for quantitative concentration, purification and separation of proteins and viral gene vectors.  Their unique truncated conical filtration chambers provide a high ratio of membrane  area to sample size. This, in turn, provides a high degree of concentration in a single  spin as well as better control of polarization and fouling at the membrane surface.  Apollo has the largest available sample volumes for a given centrifuge tube size

  • >90%  quantitative recovery of micrograms of retained protein
  • Quantative salt/buffer exchange in a single spin. 1000x plus
    concentration factor capability
  • Fast concentration of up to 20mL of protein or virus sample 
  • Biosafety    No aerosol generation or airlocking
  • No need for hand contact with wetted surfaces
  • No invert spin required

 

click here for more details

Click here for a list of Apollo Citations

 

 

Available in packs of 8, 25,100  and 250.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

APOLLO 20 mL CONCENTRATORS

Ultrafiltration using Apollo® Concentrators (US patents 6,269,957, 6,357,601) combines the highest recovery of regenerated cellulose membrane devices with faster concentration of protein samples and viral gene vectors than possible with recent high-speed devices of comparable retention. Apollo ultrafiltration devices are used for purification, concentration or desalting of proteins and viral gene vector particles. They are available in 7 mL and 20 mL versions, each having 3 different membrane retention ratings: 9 kDa, 20 kDa, and 150 kDa.

Orbital Biosciences

Click here to download protocol

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

APOLLO 20 mL CONCENTRATORS

Ultrafiltration using Apollo® Concentrators (US patents 6,269,957, 6,357,601) combines the highest recovery of regenerated cellulose membrane devices with faster concentration of protein samples and viral gene vectors than possible with recent high-speed devices of comparable retention. Apollo ultrafiltration devices are used for purification, concentration or desalting of proteins and viral gene vector particles. They are available in 7 mL and 20 mL versions, each having 3 different membrane retention ratings: 9 kDa, 20 kDa, and 150 kDa.

Orbital Biosciences

Examples of recent publications citing the use of Orbital Biosciences' Apollo concentrators:

Boss IW, Nadeau PE, Abbott JR, Yang Y, Mergia A, Renne R.  A Kaposi's sarcoma-associated herpesvirus-encoded ortholog of microRNA miR-155 induces human splenic B-cell expansion in NOD/LtSz-scid IL2Rγnull mice.  J Virol. 2011 85:9877-86.

DiMattia MA, Nam HJ, Van Vliet K, Mitchell M, Bennett A, Gurda BL, McKenna R, Olson NH, Sinkovits RS, Potter M, Byrne BJ, Aslanidi G, Zolotukhin S, Muzyczka N, Baker TS, Agbandje-McKenna M.  Structural insight into the unique properties of adeno-associated virus serotype 9.  J Virol. 2012 86:6947-58.

Doerfler PA, Byrne BJ, Clément N.  Copackaging of multiple adeno-associated viral vectors in a single production step.  Hum Gene Ther Methods. 2014 25:269-76.

Halder S, Van Vliet K, Smith JK, Duong TT, McKenna R, Wilson JM, Agbandje-McKenna M.  Structure of neurotropic adeno-associated virus AAVrh.8.  J Struct Biol. 2015 192:21-36.

Higuchi M, Haginoya K, Yamazaki T, Miyamoto K, Katagiri T, Tomimoto K, Shitomi Y, Hayakawa T, Sato R, Hori H. Binding of Bacillus thuringiensis Cry1A toxins to brush border membrane vesicles of midgut from Cry1Ac susceptible and resistant Plutella xylostella.   Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology  2007 147: 716–724.

Hori Y, Hashimoto T, Nomoto H, Hyman BT, Iwatsubo T.  Role of Apolipoprotein E in β-Amyloidogenesis: Isoform-Specific Effects on Protofibril to Fibril Conversion of Aβ in vitro and Brain Aβ Deposition in vivo.  J Biol Chem. 2015 290:15163-7.

Kuboshima M, Shimada H, Liu TL, Nomura F, Takiguchi M, Hiwasa T, Ochiai T. Presence of serum tripartite motif-containing 21 antibodies in patients with esophageal squamous cell carcinoma. Cancer Science 2006 97:380-386.

Mahajan MC, Narlikar GJ, Boyapaty G, Kingston RE, Weissman SM.  Heterogeneous nuclear ribonucleoprotein C1/C2, MeCP1, and SWI/SNF form a chromatin remodeling complex at the beta-globin locus control region.  Proc Natl Acad Sci U S A. 2005 102:15012-7. 

Méndez-Gómez, H. R., Galera-Prat, A., Meyers, C., Chen, W., Singh, J., Carrión-Vázquez, M., & Muzyczka, N. Transcytosis in the blood–cerebrospinal fluid barrier of the mouse brain with an engineered receptor/ligand system.  Molecular Therapy. Methods & Clinical Development 2015 2, 15037.

Okai M, Kudo N, Lee WC, Kamo M, Nagata K, Tanokura M.  Crystal structures of the short-chain flavin reductase HpaC from Sulfolobus tokodaii strain 7 in its three states: NAD(P)( )(-) free, NAD( )(-) bound, and NADP( )(-) bound.  Biochemistry. 2006 45:5103-10.

Pandya J, Ortiz L, Ling C, Rivers A & Aslanidi G.  Rationally designed capsid and transgene cassette of AAV6 vectors for dendritic cell-based cancer immunotherapy.  Immunology and Cell Biology 2014 92, 116–123.

Reddy VB, Iuga AO, Kounga K, Lerner EA.  Functional analysis of recombinant mutants of maxadilan with a PAC1 receptor-expressing melanophore cell line.  J Biol Chem. 2006 281:16197-201.

Reisz JA, Zink CN, King SB.  Rapid and selective nitroxyl (HNO) trapping by phosphines: kinetics and new aqueous ligations for HNO detection and quantitation.  J Am Chem Soc. 2011 133:11675-85.

Sayroo R, Nolasco D, Yin Z, Colon-Cortes Y, Pandya M, Ling C, Aslanidi G.  Development of novel AAV serotype 6 based vectors with selective tropism for human cancer cells.  Gene Ther. 2016 23:18-25.

Tsunetsugu-Yokota Y, Ato M, Takahashi Y, Hashimoto S, Kaji T , Kuraoka M, Yamamoto K, Mitsuki Y, Yamamoto T, Oshima M, Ohnishi K & Takemori T. Formalin-Treated UV-Inactivated SARS Coronavirus Vaccine Retains Its Immunogenicity and Promotes Th2-Type Immune Responses.  Jpn. J. Infect. Dis. 2007 60: 106-112.

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200