Cambio - Excellence in Molecular Biology

STEM Learning

STEM Learning: STEM Kits

A range of easy to use kits for PCR, gel electrophoresis and other molecular biology techniques especially designed for budding school students, in schools, 6th form colleges and other educational institutes.

 

5X HOT Multiplex PCR Master Mix (1 ml)

premixed, ready-to-use solution containing all reagents required for hot-start multiplex PCR – just add your template DNA, primers and water.

MiniPCR - Amplyus

Catalogue No.DescriptionPack SizePriceQty
MP-RG-1010-015X HOT Multiplex PCR Master Mix (1 ml)1ml £150.00 Quantity Add to Order
MP-RG-1021-01Nuclease free water ( 1.0 ml)1.0 ml £9.00 Quantity Add to Order

Description

5X HOT Multiplex PCR Master Mix (1 ml)

 

A premixed, ready-to-use solution containing all reagents required for hot-start multiplex PCR – just add your template DNA, primers and water. A Load-Ready master mix, this product includes an additional compound needed for direct loading onto agarose gel and two tracking dyes (blue and yellow) to allow easy progress monitoring during electrophoresis. Generated PCR products are compatible with T/A cloning procedures.

  • 1 ml vial
  • Sufficient for 250 reactions at 4 μl master mix per reaction (i.e., 20 μl total reaction volume)
  • Inhibits primer dimer formation
  • Hot-start: initial activation in 12-15 min
  • Multiplexing: tested with up to 18 targets (depending on amplicon length)
  • Amplicon size: up to 5 kb
  • Stable formulation may be shipped or temporarily stored for up to 1 month at room temperature, or up to 1 year at -20°C

Contents:

  • 5X Multiplex Buffer with 10 mM MgCl2 (in 1X PCR solution: 2 mM MgCl2)
  • Hot-start DNA polymerase
  • dNTPs (dATP, dCTP, dGTP and dTTP)
  • Bovine serum albumin (BSA) to enhance PCR reaction
  • Blue dye with migration rate equivalent to 3.5-4.5 kb DNA fragment
  • Yellow dye with migration rate equivalent to 35-45 bp DNA fragment
  • Compound that increases sample density for direct gel loading

Recommended storage: -20ºC for 12 months
May be shipped or temporarily stored for up to 1 month at room temperature without discernible loss of activity.

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