Cambio - Excellence in Molecular Biology

Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

BHQ-2 Phosphoramidite

4'-(4-Nitro-phenyldiazo)-2'-methoxy-5'-methoxy-azobenzene-4''-(N-2-4,4'-dimethoxytrityl(oxyethyl))-N-ethyl-2-cyanoethyl-(N,N-diisopropyl)-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1962-02BHQ-2 Phosphoramidite0.25grams £1,359.00 Quantity Add to Order
10-1962-90BHQ-2 Phosphoramidite100µmoles £600.00 Quantity Add to Order
10-1962-95BHQ-2 Phosphoramidite50µmoles £309.00 Quantity Add to Order

Description

Black Hole Quencher-2 (BHQ-2) Phosphoramidite dye is a non-nucleoside version that has O-4,4'-dimethoxytrityl “O-DMT”, which will allow the incorporation of this modification internally or at the 3' end of the oligonucleotide by using one of our universal supports without using the 3' CPG version. The structure of the BHQ-2 Phosphoramidite consists of a polyaromatic-azo backbone (Ph-N=N-Ph). This allows it to absorb a broad range of wavelengths in the visible spectrum, resulting in the absorption of many colors.

Product Specifications

Formula: C54H61N8O9P
M.W.: 997.10
F.W.: 556.47
CAS Number: 374591-98-7

 

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer
  • Deprotection: Complete the deprotection using the protocol required by the nucleobases. Note, the use of methylamine in ultrafast deprotection protocols will result in degradation to the dye and is not recommended.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions

LV40 LV200 40nm 0.2μm 1μm 10μm
10-1962-95 50 µmol 0.05 0.5 3.33 2 1.25 0.91 0.67 0.17
10-1962-90 100 µmol 0.1 1 20 12 7.5 5.45 4 1
10-1962-02 0.25 g 0.25 2.51 70.33 42.2 26.38 19.18 14.07 3.52

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions

Molarity 50nm 0.2μm 1μm 15μm
10-1962-95E 50 µmol 0.05 0.75 0.07 8.6 5.38 3.91 0.54
10-1962-90E 100 µmol 0.1 1.5 0.07 23.6 14.75 10.73 1.48
10-1962-02E 0.25 g 0.25 3.74 0.07 68.4 42.75 31.09 4.28

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

References

Download the following (links to manufacturer's website):

SDS

Glen Report article

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200