Cambio - Excellence in Molecular Biology

STEM Learning

STEM Learning: STEM Kits

A range of easy to use kits for PCR, gel electrophoresis and other molecular biology techniques especially designed for budding school students, in schools, 6th form colleges and other educational institutes.

 

Chopped Using CRISPR-Cas9 to cut DNA

Cut DNA in a test tube using the CRISPR/Cas9 system!

MiniPCR - Amplyus

Catalogue No.DescriptionPack SizePriceQty
MP-KT-1801-01Chopped! Using CRISPR-Cas9 to cut DNAKit £123.00 Quantity Add to Order

Description

Chopped! Using CRISPR/Cas9 to cut DNA

 

Use the CRISPR/Cas9 system in vitro to cut DNA in a tube and analyze the results with gel electrophoresis. Investigate how the Cas9 nuclease can be programmed to target virtually any DNA sequence—just by changing the guide RNA.

 

Watch the Video here

The introduction of CRISPR/Cas into the biotechnology toolkit has revolutionized gene targeting and gene editing. In this lab, students will use the CRISPR/Cas system in vitro (in a test tube) to analyze the function of its molecular components. They will use two different guide RNAs to direct Cas9 nuclease to cut a DNA sample at two different locations and then analyze their resulting DNA fragments using gel electrophoresis. This gene targeting lab will allow students to investigate how the Cas9 nuclease can be programmed to target virtually any DNA sequence—just by changing the guide RNA.  This activity will demonstrate how the CRISPR/Cas system is both programmable and specific and why it is such a powerful genome editing tool. 

  • Techniques: Micropipetting, gene targeting, gel electrophoresis
  • Topics: Genome editing, biotechnology, CRISPR/Cas9
  • Time required: Two 45-min class periods or one 90-min block
  • Level: General high school through college

 

 

What is included ?

This lab provides enough reagents for eight lab groups:

  • Nuclease-Free Water
  • Reaction Buffer
  • Cas9 Nuclease
  • Guide RNAs (gRNA1 and gRNA2)
  • DNA Sample
  • Proteinase K Solution
  • Fast DNA Ladder 3

IMPORTANT storage notes:

  • The CHOPPED! reagents will be shipped overnight on ice and will need to be stored in the freezer immediately upon arrival. We strongly recommend storing the reagents in the coldest part of the freezer where temperature fluctuations are minimal (e.g., in the back of the freezer instead of the freezer door). 
  • The Cas9 Nuclease has greater stability when stored at higher concentrations. For this reason, you will need to dilute the enzyme shortly before use. See the Instructor’s Guide for more details.
  • Reagents are stable for 12 months when stored according to these recommendations.

Required equipment:

  • Micropipettes and tips 
  • 37 °C heat source (e.g., incubator, water bath, or heat block)
  • Gel electrophoresis and fluorescence visualization system  
  • Optional: Vortex and microcentrifuge 

Reagents and consumables supplied by user:

The Learning Lab Companion Kit (catalog no. KT-1510-01) contains all the consumables (except water) needed for 8 lab groups. These reagents and consumables can also be sourced independently.

  • Agarose (electrophoresis grade)
  • DNA stain (e.g., SeeGreen™ or GelGreen®)
  • Gel electrophoresis buffer (e.g., 1X TBE)
  • Distilled or deionized H2O (to dilute buffer concentrate – not included in Lab Companion Kit)
  • Microtubes
  • PCR tubes

Downloads

 

 

 

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