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Oligo Synthesis

Oligo Synthesis : CEPs

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Ac-dC-5’-CE Phosphoramidite

3'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-5101-02Ac-dC-5'-CE Phosphoramidite0.25g £64.00 Quantity Add to Order
10-5101-05Ac-dC-5'-CE Phosphoramidite0.5g £123.00 Quantity Add to Order
10-5101-10Ac-dC-5'-CE Phosphoramidite1.0g £236.00 Quantity Add to Order

Description

Glen Research 5'-CE (ß-cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for special applications. The acetyl-protected dC is compatible with all standard deprotection methods.

Product Specifications

Formula: C41H50N5O8P
M.W.: 771.85
F.W.: 289.18
CAS Number: 1632243-18-5

 

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions

LV40 LV200 40nm 0.2μm 1μm 10μm
10-5101-02 0.25 g .25grams 3.24 94.67 56.8 35.5 25.82 18.93 4.73
10-5101-05 0.5 g .5grams 6.48 202.67 121.6 76 55.27 40.53 10.13
10-5101-10 1.0 g 1gram 12.96 418.67 251.2 157 114.18 83.73 20.93

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions

Molarity 50nm 0.2μm 1μm 15μm
10-5101-02E 0.25 g .25grams 4.83 0.07 90.2 56.38 41 5.64
10-5101-05E 0.5 g .5grams 9.67 0.07 187 116.88 85 11.69
10-5101-10E1.0 g1gram19.340.07380.4237.75172.9123.78

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References

References

The Glen Report, 1993, 6.1, 6.

The Glen Report, 2019, 31.1, 11-12.

The Glen Report, 2014, 26.1, 4-6.

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Notes

Safety Data Sheet

Glen Report 6.14: 5' To 3' Synthesis

Glen Report 34-14: New Product — Ac-dC-5’-CE Phosphoramidite

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Related products

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