5-Me-C-2'-MOE-Phosphoramidite
5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-N4-benzoyl-
cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Like the very similar 2’-OMe
backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced
duplex stability, significant nuclease resistance and relatively low
toxicity. As a result, 2’-MOE has been an attractive backbone for many
therapeutic candidates, several of which have been approved by the FDA.
The
se drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H
cleavage of target RNA sequences as well as 2) steric blocking
oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE
nucleotides are A, 5-Me-C, G and 5-Me-U.
Details
Usage
- Coupling: 6 minute coupling time recommended
- Deprotection:
No changes needed from standard method recommended by synthesizer
manufacturer. Note: Deprotection in AMA (50:50 Ammonium
hydroxide/MethylAmine) may result in a 4-5% 5-methyl-N4-methyl-C side
product at the modification site.
| Specifications |
| Diluent |
Anhydrous Acetonitrile |
| Storage |
Refrigerated storage, maximum of 2-8°C, dry |
| Stability |
1-2 Days |
Safety Data Sheet
Glen Report 32.14: New Product — 2'-MOE RNA Phosphoramidites
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