Cambio - Excellence in Molecular Biology

Transfection-Biontex

Transfection-Biontex: General transfection reagents

Broad range transfection reagents for a variety of mammalian and insect cells

Metafectene® Transfection Reagent

The Metafectene® Transfection Reagent is a highly efficient Transfection Reagent for Mammalian Cells developed by Biontex and based on RMA-Technology (Repulsive Membrane Acidolysis)

Biontex

Catalogue No.DescriptionPack SizePriceQty
T020-1.0Metafectene™1ml £181.00 Quantity Add to Order
T020-2.0Metafectene™2x1ml £331.00 Quantity Add to Order
T020-5.0Metafectene™5x1.0ml £749.00 Quantity Add to Order

Description

Highly efficient Transfection Reagent for Mammalian Cells developed by Biontex and based on RMA-Technology (Repulsive Membrane Acidolysis). 

These lipids ensure easy entry of DNA into cells by condensing DNA into compact structures. Furthermore, endosome buffering is enabled to protect against osmotic shock and destabilisation of the DNA-coated lipid membrane can be achieved. 

The high efficiency of Metafectene makes it a valuable tool for gene delivery or gene transfer experiments. It is provided as a ready-to-use solution. In contrast to many other transfection reagents Metafectene shows no serum inhibition, which makes it suitable for transfecting sensitive cell lines. 1ml is usually sufficient for 50-200 transfections on 35mm dishes (6-well plates) or 15 to 70 transfections on 60mm dishes. 

Cells which have been successfully transfected with Metafectene include: kidneys from human, porcine, baby and Syrian hamster and monkey. Human epidermal, breast, cervical, hepatoblastoma, T-cell leukaemia and lung carcinoma, mouse bone marrow, Chinese hamster ovary, human uroepithelial cells, mouse macrophage and adherent fibroblastoid, rat adrenal cytoma and hepatocytes and porcine smooth muscle cells. 

Storage

Storage at 4°C for more than a year is no problem in respect to transfection efficiency. It has also been shown to be stable for weeks at 40°C.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

Notes

Product Specifications

 
Application Transfection of mammalian cells with nucleic acids
Formulation Cationic lipids with colipids in water
Assays Approx. 500 (24-well) or approx. 200 (6-well) per 1 ml reagent
Shipping At room temperature
Storage 4°C
Shelf Life 1 year (after date of delivery)

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

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If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200