Cambio - Excellence in Molecular Biology

Extraction & Purification - DNA

Extraction & Purification - DNA: Total DNA

Fast and easy methods for the micro scale purification of total (genomic and mitochondrial) DNA from various biological samples.

QuickExtract™ FFPE DNA Extraction Solution

The QuickExtract™ FFPE DNA Extraction Solution is for the analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process.

BioSearch Tech (Lucigen/Epicentre)

Catalogue No.DescriptionPack SizePriceQty
QEF81050QuickExtract™ FFPE DNA Extraction Kit500 rxns £248.00 Quantity Add to Order

QuickExtract™ FFPE DNA Extraction Solution

The QuickExtract™ FFPE DNA Extraction Solution is for the analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process.

BioSearch Tech (Lucigen/Epicentre)

The analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process. These challenges include chemical modification of the DNA, cross-linking of DNA with other molecules, degradation of the DNA, and the limited amount of nucleic acid in the samples. 

The QuickExtract™ FFPE DNA Extraction Kit is a fast, simple, and inexpensive method for preparing genomic DNA for PCR amplification from archival samples. The protocol requires only heat treatment to melt the paraffin, lyse the cells, decrease the fomalin-induced cross-linking in the sample, and degrade compounds inhibitory to amplification. Following heat treatment, the sample DNA is ready for PCR. This product is only intended for PCR-based applications. If DNA is required for other molecular biology applications, consider the MasterPure™ DNA Purification Kit.

 

 

Quickextract
fig 1 Figure 1. PCR amplification of DNA from a slide-mounted, FFPE-preserved human skeletal muscle tissue section, extracted with the QuickExtract™ FFPE DNA Extraction Kit.
Two microliters of undiluted, extracted DNA was amplified with primers for three different loci: tumor protein 53 (TP53), dystrophin (DMD), and tumor necrosis factor (TNF). The products were separated on a 3% agarose gel and were visualized with SYBR® Gold. Lane M, 100 bp DNA ladder; Lane 1, exon 2 of TP53; Lane 2, exon 3 of TP53; Lane 3, exon 11 of TP53; Lane 4, exon 6 of DMD; Lane 5, exon 50 of DMD; Lane 6, exon 3 of DMD; Lane 7, exon 4 of TNF.
fig 2 Figure 2. Short Tandem Repeat (STR) PCR amplification of DNA from slide-mounted, FFPE-preserved, human tissue sections, combined and extracted with the QuickExtract™ FFPE DNA Extraction Kit.
Two microliters of undiluted, extracted DNA was amplified with primers for seven different STR loci. The products were separated on a 3% agarose gel and were visualized with SYBR® Gold. The expected allele-to-allele variation in STR sequences results in multiple products per primer set. Lane M, 100 bp DNA ladder; Lane 1, RENA4; Lane 2, D3S1358; Lane 3, D7S820; Lane 4, THO1; Lane 5, D19S253; Lane 6, D21S11; Lane 7, AMEL.




If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

QuickExtract™ FFPE DNA Extraction Solution

The QuickExtract™ FFPE DNA Extraction Solution is for the analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process.

BioSearch Tech (Lucigen/Epicentre)

Protocols for: QuickExtract™ FFPE DNA Extraction Kit

protocol

(catalogue number QEF81805 / QEF81050)

Please note: all protocols off site are the responsibility of the products supplier

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

QuickExtract™ FFPE DNA Extraction Solution

The QuickExtract™ FFPE DNA Extraction Solution is for the analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process.

BioSearch Tech (Lucigen/Epicentre)

 

 

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

QuickExtract™ FFPE DNA Extraction Solution

The QuickExtract™ FFPE DNA Extraction Solution is for the analysis of nucleic acid from formalin-fixed, paraffin-embedded (FFPE) specimens is challenging due to the extensive cross-linking of all tissue components during the fixation process.

BioSearch Tech (Lucigen/Epicentre)

Applications

  • Isolation of DNA from FFPE samples for PCR-based analysis, e.g., microsatellite detection, SNP detection, tumor heterogeneity studies, copy number detection, methylation analysis, and Short Tandem Repeat (STR) analysis. 

Benefits

  • PCR-ready DNA in minutes, not days.
  • No xylene or phenol extraction.
  • No columns, transfers, or sample loss.
  • Extracted DNA is compatible with both real-time and endpoint PCR.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200