Pfu DNA Polymerase 2x master mix is ready to use premix which contains Pfu DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. It has been optimized for routine PCR applications. Pfu DNA Polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures (1). Pfu 2x Master Mix product is supplied with the unique Intact Genomics 5x Magic Enhancer that enables efficient amplification of GC rich templates up to 84%.
Product Description
Product Source
E. coli strain expressing a Pfu DNA Polymerase gene from Pyrococcus furiosus.
Pfu DNA Polymerase Comparison Data
We repeatedly compare our Pfu Polymerase side-by-side with a leading competitor for PCR assay. Our enzyme is better than the competitor. A typical gel picture is shown below:
Applications
- Routine PCR
- Primer extension
- Colony PCR
- Genotyping
- Efficient for amplifying high GC content template DNA with Magic Enhancer
Product Includes
- Pfu 2x master mix
- 5x Magic Enhancer
Storage Temperature
–20 °C
1x Master Mix Composition
10 mM Tris-HCl pH 9.0, 50 mM KCl, 1.5 mM MgCl2, 0.2 mM dNTPs, 5% Glycerol, 0.08% Igepal CA 630, 0.05% Tween-20, 100 Units/ml Pfu DNA Polymerase.
Protocol
1. Prepare a reaction mix according to the following table:
2. Mix the reaction mixture thoroughly.
3. Program the thermal cycler according to the manufacturer’s instructions.
4. A typical PCR cycling program is outlined in the following table.
5. Place the PCR tubes in the thermal cycler and start the cycling program.
6. Analyze 5 μl of PCR products by agarose gel electrophoresis.
Reference
1. Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.
Downloads
Manual – Pfu 2x Master Mix
MSDS – ig Pfu 2x Master Mix