qPCR and qRT-PCR: IG® PCR and qPCR Enzymes, Master Mix, and Kits

IG® PCR and qPCR Enzymes, Master Mix, and Kits

ntact Genomics (IG®, ig®) develops and manufactures high-quality enzymes, master mixes, and kits for conventional PCR, high-fidelity PCR, quantitative PCR (qPCR), genotyping, direct PCR, and nucleic acid sample preparation.

Our portfolio includes dye-based and probe-based qPCR master mixes, high-fidelity DNA polymerases, Taq and hot-start Taq formulations, ready-to-use PCR master mixes, Exonuclease I, and FastAmp® sample-preparation and direct-PCR solutions. These products are designed to deliver reliable amplification, high sensitivity and specificity, consistent performance, and convenient workflows across a broad range of research and molecular-testing applications.

Why Choose IG® PCR and qPCR Products?

Reliable Performance – Optimized for consistent amplification, sensitivity, specificity, and reproducibility.
Solutions for Diverse Applications – Products for routine PCR, high-fidelity amplification, qPCR, genotyping, direct PCR, and post-PCR primer cleanup.
Convenient Formats – Choose individual enzymes or ready-to-use master mixes to simplify setup and reduce pipetting errors.
Streamlined Sample Preparation – FastAmp® solutions enable rapid sample lysis and direct amplification without conventional DNA or RNA purification.
Consistent Quality – Manufactured under an ISO 13485:2016-certified quality management system.
Flexible Supply Options – Bulk quantities, custom formulations, private-label products, and OEM partnerships are available upon request.

Explore our PCR and qPCR product portfolio(opens in new tab) to select the right enzyme, master mix, or kit for your application.

Product Links	Size Available	Application & Benefits
IG® Green qPCR 2X Master Mix	200, 500 or 2,000 Reactions	Ready-to-use qPCR cocktail containing all components except primers and template.
igScript™ Probe-Based qPCR Master Mix	500, 1000, or 2,500 Reactions	Provides improved PCR efficiency, wider dynamic range, superior sensitivity, and specificity.
i7® High-Fidelity DNA Polymerase	200 or 500 Units	High fidelity, sensitivity, and processivity with an error rate approximately 280× lower than Taq DNA Polymerase.
i7® High-Fidelity DNA Polymerase 2X Master Mix	100 or 500 Reactions	Convenient master mix format of our high-fidelity polymerase.
Pfu 2X Master Mix	200 Reactions	Affordable and robust high-fidelity polymerase.
Taq DNA Polymerase	1,000 or 5,000 Units	For robust and affordable PCR.
Taq DNA Polymerase 2X Premix	500 or 1,000 Reactions	Convenient master mix format of our robust Taq DNA Polymerase.
Hot Start Taq 2X Master Mix	500 Reactions	Reduces non-specific amplification during setup and improves PCR specificity and sensitivity.
Exonuclease I (Exo I)(opens in new tab)	5,000 Units or 20,000 Units	Removes residual primers while preserving dsDNA.
FastAmp® Plant Direct PCR Kit	250 or 1,250 Reactions	Amplifies DNA directly from plant samples without DNA purification.
FastAmp® Plant Direct PCR & Genotyping Solution	20 mL	Rapid cell lysis and DNA separation without the need for DNA extraction.
FastAmp® Viral and Cell Lysis Solution	100 Reactions (50 mL) or 500 Reactions (250 mL)	Streamlines sample collection, storage, and amplification by isothermal RPA.

IGScript™ Probe-Based qPCR master mix

igScript™ Probe-Based qPCR Master Mix delivers sensitive, specific, and reproducible real-time PCR amplification and quantification. Its optimized, ready-to-use formulation simplifies assay setup and supports reliable performance across gene-expression analysis, genotyping, pathogen detection, and other molecular research applications.

Intact Genomics

Catalogue No.	Description	Pack Size	Price	Qty	Change to:
IG-4233	IGScript™ Probe-Based qPCR master mix	500 Reactions (20 µl rxn vol)	�286.52	Quantity	Add
IG-4235	IGScript™ Probe-Based qPCR master mix	1000 reactions (20 µl rxn vol)	�425.72	Quantity	Add
IG-4235	IGScript™ Probe-Based qPCR master mix	1000 reactions (20 µl rxn vol)	�803.88	Quantity	Add
IG-4235	IGScript™ Probe-Based qPCR master mix	1000 reactions (20 µl rxn vol)	�171.68	Quantity	Add

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GScript™ Probe-Based qPCR master mix

Product Description

IgScript™ Probe-Based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x master mix is a ready-to-use cocktail containing all components except primers, probe and template, for the amplification and detection of DNA in qPCR. This 2x master mix requires minimal handling during reaction setup and offer consistent and robust qPCR reactions. Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase (1, 2) and a 5´→3´ exonuclease activity (3, 4). The amplification step features a high quality Taq DNA Polymerase which offers robust, reliable and better amplification.

Applications

Gene expression data validation.
Multiplexing
Mutation detection
Pathogen and viral detection
Genetically modified organisms (GMO) characterization and Genetic profiling

Benefits

Enhanced efficiency, specificity, and sensitivity
Compatible with all real-time PCR instruments
Superior gene expression results under various cycling conditions

Product Components and Storage

igScript™ Probe-Based qPCR master mix
Store at -20 °C

Technical Support

Intact Genomics is committed to supporting the worldwide scientific research community by supplying the highest quality reagents. Each new lot of our products is tested to ensure they meet the quality standards and specifications designated for the product. Please follow the instructions carefully and contact us if additional assistance is needed. We appreciate your business and your feedback regarding the performance of our products in your applications.

Protocol

1. Place kit components and DNA samples on ice.
2. Mix and then centrifuge briefly to collect contents at the bottom of the tube.
3. Prepare a master mix for each reaction and control requiring Reverse Transcriptase enzyme plus 10% extra to allow for pipetting error according to the following table:

4. Mix the reaction mixture thoroughly.
5. Program the thermal cycler according to the manufacturer’s instructions.
6. A typical PCR cycling program is outlined in the following table.

7. Place the PCR tubes in the thermal cycler and start the cycling program.
8. Analyze the data according to manufacturer protocol.

* For 3 step cycling protocols, anneal at optimal annealing temperature for 30 sec followed by the minimum time required for data acquisition at 72 ºC according to instrument guidelines.

Downloads

igScript™ Probe-Based qPCR master mix Manual
igScript™ Probe-Based qPCR Master Mix MSDS

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

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Buffers (custom)