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Competent Cells

Competent Cells: IG® Ultra-High Efficiency Electrocompetent E. coli Cells

IG® Ultra-High Efficiency Electrocompetent E. coli Cells

 

Intact Genomics (IG®, ig®) offers the largest collections of high-quality competent cells on the market.

Originally founded as a BAC cloning company, Intact Genomics developed deep expertise in producing ultra-high-efficiency competent cells to enable transformation of very large plasmids (~300 kb) into E. coli. Over the years, this expertise—combined with continuous process optimization—has allowed us to consistently deliver exceptionally high transformation efficiencies and reliable performance.

Combining our scientists’ expertise and craftsmanship with state-of-the-art equipment, advanced protocols, and rigorous quality control standards, our competent cells provide the following advantages:

  • Exceptional Transformation Efficiency – Up to 10–100× higher than other leading brands.
  • Consistent Quality – Manufactured under an ISO 13485:2016-certified quality management system to ensure reliable lot-to-lot performance.
  • Competitive Pricing– High-performance products at affordable prices to help maximize your research budget.
  • Flexible Formats and Custom Services – We offer a broader range of formats than competitors, including tubes (multiple aliquot sizes), 96- and 384-well plates, and strip tubes. We also provide custom competent cell services tailored to your specific needs.

Application

Product 

Specialization

Efficiency

Routine Cloning

ig® 5-alpha Electrocompetent Cells

Routine cloning and sub-cloning, library construction (DNA <10 kb)

≥2.0 × 1010

ig® 10B Electrocompetent Cells

Routine cloning and sub-cloning, library preparation including large DNA >10 kb

≥5.0 × 1010

ig® XL1 Blue Max Electrocompetent Cells

Stable plasmid propagation with blue/white screening

≥5.0 × 1010

HB101 Electrocompetent Cells

Stable plasmid maintenance

≥5.0 × 1010

Specialized Cloning

igMax™ DH10B Electrocompetent Cells

Difficult large DNA fragment cloning, BAC library construction

≥5.0 × 1010

IG® Recombineering Electrocompetent Cells

Homologous recombination, DNA, plasmid, and BAC engineering

≥1.0 × 1010

ig® Stable 2 Electrocompetent E. coli Cells

Cloning direct repeat sequences (unstable DNA)

≥2.0 × 109

ig® ccdB Resist™ Electrocompetent E. coli Cells

Propagation of plasmids containing the ccdB gene

≥1.0 × 1010

Staphylococcus aureus (S. aureus) RN4220 ElectroCompetent Cells

A gateway strain to enable DNA manipulation in Staphylococcus aureus

≥1 × 105

Protein Expression

IG® Autoinduction DE3 ElectroCompetent Cells

Optimized for autoinduction of protein expression

≥1.0 × 1010

BL21(DE3) Electrocompetent Cells

Routine T7 protein expression

≥1.0 × 1010

BL21(DE3)pLysS ElectroCompetent Cells

Routine non-T7 protein expression

≥1.0 × 1010

IG® C41(DE3) Electrocompetent Cells

Improved expression of toxic proteins

≥1.0 × 1010

IG® C41(DE3)pLysS Electrocompetent Cells

Improved expression of toxic proteins with suppressed basal expression

≥1.0 × 109

IG® C43(DE3) Electrocompetent Cells

Most tolerant of toxic proteins

≥1.0 × 1010

IG® C43(DE3)pLysS Electrocompetent Cells

Most tolerant of toxic proteins with suppressed basal expression

≥5.0 × 109

Phage Display

TG1 Phage Display Electrocompetent Cells

Antibody phage display with amber suppressor

≥4.0 × 1010

SS320 Phage Display Electrocompetent Cells

Antibody phage display

≥5.0 × 1010

ER2738 Phage Display ElectroCompetent Cells

Peptide phage display with amber suppressor

≥4.0 × 1010

Medium

IG® Recovery Medium

Superior formulation for all competent cell transformations

High-efficiency

IG® Autoinduction Medium

Promotes high-yield growth of E. coli and high-level expression of T7 promoter-regulated recombinant proteins

High-efficiency
 

Custom Formats, Custom Package Sizes and Bulk Pricing Are Available Upon Request

 

Staphylococcus Aureus (S. aureus) RN4220 Electrocompetent Cells

Staphylococcus aureus RN4220 strain is commonly used in studies involving virulence, resistance, metabolism and more.

Intact Genomics

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
IG-1294-20Staphylococcus Aureus (S. aureus) RN4220 Electrocompetent Cells10x50µl �467.48 Quantity
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IG-1294-40Staphylococcus Aureus (S. aureus) RN4220 Electrocompetent Cells5x 200µl �809.68 Quantity
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Staphylococcus Aureus (S. aureus) RN4220 Electrocompetent Cells

 

 

Staphylococcus aureus RN4220 strain is commonly used in studies involving virulence, resistance, metabolism and more.

 

Product Description

Intact Genomics (ig®) Staphylococcus aureus (S. aureus) RN4220 Electrocompetent Cells are optimized to provide high transformation efficiencies making them ideal for various applications. This strain is commonly used in studies involving virulence, resistance, metabolism and more. Staphylococcus aureus RN4220 is characterized by a mutation in the sau1 hsdR genes, making it restriction deficient and hence an excellent intermediate cloning host.

 

Specifications

Competent cell type: Electrocompetent
Species: S. aureus
Strain: RN4220
Format: Tubes
Transformation efficiency: ≥1 x 105 cfu/µg DNA
Blue/white screening: No
Shipping condition: Dry ice

 

Reagents Included

ig® Staphylococcus aureus (S. aureus) RN4220 Electrocompetent Cells
Recovery medium

 

Storage

ig® Staphylococcus aureus (S. aureus) RN4220 Electrocompetent Cells: -80 ºC
Recovery medium: 4 ºC

 

Quality Control

Transformation efficiency is tested by using the control DNA supplied with the kit and using the high-efficiency transformation protocol. Transformation efficiency should be equal or greater than 1×105 CFU/µg DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.

 

General Guidelines

Follow these guidelines when using ig® S. aureus Electrocompetent Cells:
• Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical
lysis caused by pipetting.
• Thaw competent cells on ice and transform cells immediately following thawing. After adding DNA,
mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.

 

Biosafety Level: 2

Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories. 5th ed. Washington, DC: U.S. Government Printing Office, 2009; see:
www.cdc.gov/biosafety/publications/bmbl5/index.htm.

BSL2 facility is required for purchase and use of this product.

 

Transformation Protocol
Use this procedure to transform ig® Staphylococcus aureus ElectroCompetent Cells. Do not use these cells for chemically transformation.
1. Place sterile cuvettes and microcentrifuge tubes on ice.
2. Remove competent cells from the -80 °C freezer and thaw completely on wet ice (10-15 minutes).
3. Aliquot 1 µl (10pg -1 µg) of DNA to the chilled microcentrifuge tubes on ice.
4. When the cells are thawed, add 25 μl of cells to each DNA tube on ice and mix gently by tapping 4-5 times. For the control, add 1 µl of (50 ng/µl) DNA to the 25 µl of cells on ice. Mix well by tapping. Do not pipette up and down or vortex to mix, this can harm cells and decrease transformation efficiency.
5. Pipette 26 µl of the cell/DNA mixture into a chilled electroporation cuvette without introducing bubbles. Quickly flick the cuvette downward with your wrist to deposit the cells across the bottom of the well and then electroporate.
6. Immediately add 500µl of Recovery Medium or any other medium of choice to the cuvette, pipette up and down three times to re-suspend the cells. Transfer the cells and Recovery Medium to an Eppendorf tube.
7. Incubate tubes at 37 °C for 1 hours at 200 RPM.
8. Dilute the cells as appropriate then spread 20-200 μl cells onto a pre-warmed selective plate. For the control plasmid , you may plate 100 μl of undiluted transformation mix onto a YT plate containing chloramphenicol 12.5µg/µl). Use sterilized spreader or autoclaved ColiRoller™ plating beads to spread evenly.
9. Incubate the plates for overnight at 37 °C.

Electroportation Settings
Mode: Exponential protocol
Voltage (V): 1,800 V
Capacitance: 25 uFD
Resistance: 200 Ohms
Cuvette: 1 mm

 

Download

Manual – Staphylococcus aureus (S. aureus) RN4220 ElectroCompetent Cells

MSDS – Staphylococcus aureus (S. aureus) RN4220 Electrocompetent Cells

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200