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Oligo Synthesis

Oligo Synthesis : Click Chemistry

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TIPS-5-Ethynyl-dU-CE Phosphoramidite

TIPS-5-Ethynyl-dU-CE Phosphoramidite 5'-Dimethoxytrityl-5-triisopropylsilyl-ethynyl-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1555-02TIPS-5-Ethynyl-dU-CE Phosphoramidite0.25 g £780.00 Quantity Add to Order
10-1555-90TIPS-5-Ethynyl-dU-CE Phosphoramidite100 umole £296.00 Quantity Add to Order
10-1555-95TIPS-5-Ethynyl-dU-CE Phosphoramidite50 umole £156.00 Quantity Add to Order

TIPS-5-Ethynyl-dU-CE Phosphoramidite

TIPS-5-Ethynyl-dU-CE Phosphoramidite 5'-Dimethoxytrityl-5-triisopropylsilyl-ethynyl-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

5-Ethynyl-dU offers convenient click conjugation with an azide to generate a label rigidly attached to one of the oligonucleotide bases.   5-Ethynyl-dU is subject to base-catalyzed hydration during cleavage and deprotection, especially when using a strong base or heat.  Hydration of an ethynyl group forms a methyl ketone which subsequently blocks potential click reactions.  Mild deprotection conditions are necessary when using 5-Ethynyl-dU-CE Phosphoramidite to prevent this side reaction.  TIPS-5-Ethynyl-dU-CE Phosphoramidite, containing a protected alkyne, offers broader compatibility with oligonucleotide synthesis and deprotection.  Protecting the 5-ethynyl group with a triisopropylsilyl (TIPS) protecting group prevents acid or base catalyzed hydration during oligonucleotide synthesis and workup.  A quick treatment with TBAF removes the TIPS protecting group.

 

M.W.: 911.15 
F.W.: 314.19 

Diluent: Anhydrous Acetonitrile

Coupling: 3 minute coupling time recommended

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. TIPS removal: After deprotection, dry down oligo and take up in 0.5 mL DMF. Add 0.1 mL TBAF. Vortex and keep at 45°C for 15 minutes. Quench with 0.5 mL 2 M TEAA and desalt on Glen Gel-Pak™ column or equivalent.

Storage: Storage: Refrigerated storage, maximum of 2-8°C, dry

Stability in Solution: 1-2 Days

Please Note: All products of baseclick are patent protected and available in collaboration with baseclick.  Baseclick GmbH has filed the following patent applications: 1. WO2006/117161, New labelling strategies for the sensitive detection of analytes. 2. WO2008/952775, Click Chemistry for the production of reporter molecules. Baseclick GmbH holds a worldwide license for the research market of the "Click Chemistry" patent from "The Scripps Research Institute": 3. WO03/101972, Copper-catalysed ligation of azides and acetylenes.

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TIPS-5-Ethynyl-dU-CE Phosphoramidite

TIPS-5-Ethynyl-dU-CE Phosphoramidite 5'-Dimethoxytrityl-5-triisopropylsilyl-ethynyl-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

MSDS

 

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

TIPS-5-Ethynyl-dU-CE Phosphoramidite

TIPS-5-Ethynyl-dU-CE Phosphoramidite 5'-Dimethoxytrityl-5-triisopropylsilyl-ethynyl-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Glen Research

Glen Report 28.2: New Product - TIPS-5-Ethynyl-dU-CE Phosphoramidite

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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200