Product Highlights 

CviJ I

Cambio

CviJ I is the only available restriction enzyme recognising two/three basepair sequence, thus allowing for new research applications. This version of CviJ I cleaves only PuGCPy sites. It does not exhibit ‘star’ activity, thus it is better suited for mapping of short DNAs like PCR products or small plasmids. Other CviJ I applications, like shotgun cloning, thermal cycle labelling or epitope mapping, can be performed either with this version or with CviJ I*, also available from Cambio. 

E.coli RNA Polymerase Core Enzyme
E.coli RNA Polymerase Sigma-saturated

Epicentre Biotechnologies 

Both the Core Enzyme and the Holoenzyme preparations of E.coli RNA Polymerase are isolated from the rifampicin-sensitive strain BL21. Epicentre® is the only company that offers purified Core Enzyme, which has no detectable sigma subunit, and 100% sigma-saturated (α70)-Holoenzyme.

The Core Enzyme is useful in studying mechanisms of transcription initiation, since it will not initiate specific transcription at promoter sequences on bacterial or bacteriophage DNA due to a lack of sigma factor. However, the Core Enzyme will generate a variety of nonspecific transcripts. The sigma-saturated Holoenzyme is very efficient in transcribing specifically a variety of double-stranded DNA templates containing promoters.

Circligase

Epicentre Biotechnologies

 CircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i.e. circularization) of single-stranded DNA (ssDNA) templates having a 5'-phosphate and a 3'-hydroxyl group. In contrast to T4 DNA Ligase and Ampligase® DNA Ligase, which ligate DNA ends that are annealed adjacently to each other on a complementary DNA sequence. CircLigase™ ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence.