Detect foodborne pathogens by semi-quantitative qPCR or conventional PCR with easy interpretable lateral flow evaluation.
Features
• Salmonella enterica – invasion protein (invA) gene
• Yersinia enterocolitica – heat-stable enterotoxin A gene
• Shigella spp. – invasion plasmid antigen (ipaH6) gene
• Campylobacter spp. – acyl-[acyl-carrier-protein]--UDP-Nacetylglucosamine O-acyltransferase (lpxA) gene
• Clostridium perfringens – phospholipase C alpha toxin (plc) gene
• Shiga Toxin 1 – stx1 gene
• Shiga Toxin 2 – stx2 gene
• Escherichia coli O157 – wbdR gene
• Escherichia coli O104 – wckD gene
• Listeria spp. – invasion associated protein p60 (iap) gene
• Listeria monocytogenes – listeriolysin O (hly) gene
• Salmonella spp. – spacer-region between 16S and 23S RNA genes
Sensitivity
Down to 10 DNA copies/assay.
Principle
The assay is based on a one tube amplification with a conventional PCR cycler and a subsequent probe specific hybridization technique. The resulting interpretation is a simple readout of coloured bars on a lateral flow stripe.
Content
PCR Mix
Rehydration Buffer
PCR Grade Water
Positive Control
Species Primer
Species Probe
LFA Stripe
LFA Running Buffer
Sample Requirements
The Food Control™ kits are designed for the PCR based detection of bacterial DNA. Isolated total DNA from potentially contaminated food serves here as starting material, typically after pre-cultivation of the sample growth medium.
Intended Use
For research use only!
Time to result
150 minutes
Cycler
Any conventional PCR cycler
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