Technical Help - Paints Protocols


Protocols

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Star*FISH©Whole Chromosome Paint

Metaphase - All Species
Hybridisation Protocol

Biotin Labelled Chromosomes
Detected using Cy3 (1043-KB-50)
Detected using Cy5 (1596-KC-50)
Detected using Texas Red (1082-KT-50)
Detected using FITC (1089-KB-50)

FITC Labelled Chromosomes
FITC Amplification Kit (1084-KF-50)

Cy3 Labelled Chromosomes
Direct labelling)

Dual Colour Kits
Biotin-Cy3 + FITC (1597-KD-50)
Biotin-Texas Red+ FITC (1090-KD-50)
FITC + Cy3 (1084-KF-50)

Sperm - Bovine X-Biotin/Y-Cy3
Hybridisation and detection protocols

Multicolour FISH© (M -FISH)
Human M-FISH© (1513-MFK) and Mouse M-FISH© (1703-MMF)

Rainbow*FISH©
Human RainbowFISH©(CA-1600) and Mouse RainbowFISH(CA-1601)

Harlequin*FISH©
Harlequin*FISH© (CA-1613)

Combined Whole and Pan Centromeric Chromosome Paints
Protocol G0 - Human and Mouse

Pan Centromeric Chromosome Paints
Protocol G1 - Human (1695) and Mouse (1697)

Chromosome-Specific Paint
Protocol G2 - Human (CPBR)

Pan Telomeric
Protocol N (1696)

PRINS Kit
Protocol P (1490-T)

Chromotrax Paints - Metaphase Chromosomes
Biotinylated p, q, pter, qter and band probes
Digoxigenin p,q, pter,qter band probes

Paraffin-Embedded Tissue
Hybridisation and detections protocols - Human and Mouse

Troubleshooting

Tavi fish website
Octavian Henegariu's FISH Guide and Troubleshooting Page

Please note that these protocols are independent and not tested directly with Cambio's products. This link is provided for your information only, and is not directly endorsed by Cambio Ltd.
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