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Oligo Synthesis

Oligo Synthesis : CEPs

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GAC Trimer Phosphoramidite

GAC Trimer Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
13-1201-90GAC Trimer Phosphoramidite 100µmoles £680.00£646.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
13-1201-95GAC Trimer Phosphoramidite 50µmoles £340.00£323.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

GAC Trimer Phosphoramidite

GAC Trimer Phosphoramidite

Glen Research

GAC Trimer Phosphoramidite

Structure

Catalog Number: 13-1201-xx

Description: GAC Trimer Phosphoramidite

5'-Dimethoxytrityl-N2-isobutyryl-2'-deoxyGuanosine-3'->5'-o-chlorophenyl-
phosphoryl-N6-benzoyl-2'-deoxyAdenosine-3'->5'-o-chlorophenyl-phosphoryl-N4-
benzoyl-2'-deoxyCytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C89H92Cl2N15O21P3 M.W.: 1869.5

Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Coupling:15 minute coupling time recommended.
Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

EXAMPLE OF PREPARATION OF TRIMER MIXTURE

Prepare 530 mg of the trimer mix, taking the amount (mg) for each trimer from the right column. Dissolve the trimer mix in dichloromethane (highest grade possible; acid-free). Evaporate to dryness to produce a homogenous mixture of all 20 trimers.

EXAMPLE OF PREPARATION OF TRIMER MIXTURE FOR THE SYNTHESIZER

Dissolve 530 mg, which is equivalent to 20X10 µmoles (normalized for RF) of the trimer mix in 2.0 mL of acetonitrile-dichloromethane mixture, 1:3 v/v to produce a 0.10N solution of trimers, ready for use in a synthesizer.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

GAC Trimer Phosphoramidite

GAC Trimer Phosphoramidite

Glen Research

GAC Trimer Phosphoramidite

GAC Trimer Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
13-1201-90 100µmoles .187grams 1 20 12 7.5 5.45 4 1
13-1201-95 50µmoles .093grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
13-1201-90 100µmoles .187grams 1.5 .07 23.6 14.75 10.73 1.48
13-1201-95 50µmoles .093grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
13-1201-90 100µmoles .187grams 1.5 .07 25.2 15.75 11.45

13-1201-95 50µmoles .093grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200