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Oligo Synthesis

Oligo Synthesis : CEPs

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GAA Trimer Phosphoramidite

GAA Trimer Phosphoramidite

Glen Research

GAA Trimer Phosphoramidite

Structure

Catalog Number: 13-1200-xx

Description: GAA Trimer Phosphoramidite

5'-Dimethoxytrityl-N2-isobutyryl-2'-deoxyGuanosine-3'->5'-o-chlorophenyl-
phosphoryl-N6-benzoyl-2'-deoxyAdenosine-3'->5'-o-chlorophenyl-phosphoryl-
N6-benzoyl-2'-deoxyAdenosine-3'-(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C90H92Cl2N17O20P3 M.W.: 1893.5

Diluent: Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v)
Coupling:15 minute coupling time recommended.
Deprotection: 30% NH4OH for 17 hours at room temperature followed by an additional 4 hours at 55°C.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

EXAMPLE OF PREPARATION OF TRIMER MIXTURE FOR THE SYNTHESIZER

Dissolve 530 mg, which is equivalent to 20X10 µmoles (normalized for RF) of the trimer mix in 2.0 mL of acetonitrile-dichloromethane mixture, 1:3 v/v to produce a 0.10N solution of trimers, ready for use in a synthesizer.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

GAA Trimer Phosphoramidite

GAA Trimer Phosphoramidite

Glen Research

GAA Trimer Phosphoramidite

GAA Trimer Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
13-1200-90 100µmoles .189grams 1 20 12 7.5 5.45 4 1
13-1200-95 50µmoles .095grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
13-1200-90 100µmoles .189grams 1.5 .07 23.6 14.75 10.73 1.48
13-1200-95 50µmoles .095grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
13-1200-90 100µmoles .189grams 1.5 .07 25.2 15.75 11.45

13-1200-95 50µmoles .095grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200