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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

Fmoc Amino-Modifier C6 dT

Fmoc Amino-Modifier C6 dT

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1536-02Fmoc Amino-Modifier C6 dT 0.25g £288.00£264.96Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1536-90Fmoc Amino-Modifier C6 dT 100µmoles £144.00£132.48Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

Fmoc Amino-Modifier C6 dT

Fmoc Amino-Modifier C6 dT

Glen Research

Fmoc Amino-Modifier C6 dT

Structure

Catalog Number: 10-1536-xx

Description: Fmoc Amino-Modifier C6 dT

5'-Dimethoxytrityl-5-[N-((9-fluorenylmethoxycarbonyl)-aminohexyl)-3-acrylimido]-
2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C63H73N6O11P M.W.: 1121.28 F.W.: 458.41(NH2)

Diluent: Anhydrous Acetonitrile
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: To label amine on column, treat support successively with 1 ml each for 10 minutes, 10% diethylamine/ACN (to remove cyanoethyl protection) and 20% piperidine/DMF to remove Fmoc protection. Rinse support with DMF and ACN to remove piperidine. Dissolve NHS ester (5-10 fold excess) in 0.5 ml appropriate solvent containing 1% diisopropylethylamine and incubate for 2-4 hours @ 35 oC with CPG to label amine. Rinse support to remove excess label and cleave/deprotect as normal or with conditions compatible with coupled label.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

SEQUENCE MODIFIERS

Sequence Modifiers are designed for use in automated synthesis. The carboxy-dT is hydrolyzed during deprotection and can be coupled directly to a molecule containing a primary amino group by a standard peptide coupling or via the intermediate N-hydroxysuccinimide (NHS) ester. Amino-Modifier dA, Amino-Modifier dC, Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis. Corresponding Amino-Modifier supports can replace their respective deoxynucleoside supports. After deprotection, the primary amine on the C6 analogues is separated from the oligonucleotide by a spacer arm with a total of 7 -10 atoms and can be labelled or attached to an enzyme. The C2 analogue is more suitable for the attachment of molecules designed to react with the oligonucleotide.

Our repertoire of NHS ester derivatives has been expanded to include the NHS-Carboxy-dT-CE Phosphoramidite. By making a dT analog of the Carboxy-Modifier C10, it is possible to label one or multiple sites within an oligonucleotide. This opens up the possibility to label any number of different dyes or molecules within an oligonucleotide when the phosphoramidite is unavailable. Doing so is straightforward and may be done manually off the synthesizer or even in a fully-automated manner on the DNA synthesizer.

We have never found conditions which allow the TFA group to be removed from an amino-modifier while the oligonucleotide remains attached to the support. We are able to solve this problem by using a 9-fluorenylmethoxycarbonyl (Fmoc) protecting group. The Fmoc group is removed using a two step procedure, the first to remove the cyanoethyl protection groups and flush the formed acrylonitrile from the synthesis column using 1% diisopropylamine in acetonitrile, and the second to remove the Fmoc group using 10% piperidine in DMF. The amino group so formed on the column can be reacted with a variety of activated esters. We offer Fmoc-Amino-Modifier C6 dT Phosphoramidite as a nucleosidic option and Amino-Modifier Serinol Phosphoramidite as a non-nucleosidic alternative. We also offer S-Bz-Thiol-Modifier C6-dT to join the ranks of thiol-modifiers for oligonucleotide synthesis. Thiol-Modifier C6-dT can be added as usual at the desired locations within a sequence.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Fmoc Amino-Modifier C6 dT

Fmoc Amino-Modifier C6 dT

Glen Research

Material Safety Data Sheet

Glen Report 19.2: NEW PRODUCTS - 2,6-Diaminopurine-TOM AND Fmoc-Amino-Modifier C6 dT Amidites

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Fmoc Amino-Modifier C6 dT

Fmoc Amino-Modifier C6 dT

Glen Research

Technical Note

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Fmoc Amino-Modifier C6 dT

Fmoc Amino-Modifier C6 dT

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.


ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1536-90 100µmoles .112grams 1 20 12 7.5 5.45 4 1
10-1536-02 0.25grams .25grams 2.23 61 36.6 22.88 16.64 12.2 3.05
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1536-90 100µmoles .112grams 1.5 .07 23.6 14.75 10.73 1.48
10-1536-02 0.25grams .25grams 3.33 .07 60.2 37.63 27.36 3.76
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1536-90 100µmoles .112grams 1.5 .07 25.2 15.75 11.45

10-1536-02 0.25grams .25grams 3.33 .07 61.8 38.63 28.09

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200