FREQUENTLY ASKED TECHNICAL QUESTION
QUESTION: What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?
RESPONSE:1. Adamantantanecarbonyl chloride - 2% w/v in 5%pyridine/acetonitrile.
2. Isopropyl phosphite - 1% in acetonitrile/pyridine (50:50)
3. Oxidation: Following synthesis
Step 1: React support with 4% Iodine in THF/Pyridine/Water ( 8:1:1) ~2mL for 4minutes
Step 2: Remove solution from support
Step 3: React support with mixture of 4% Iodine in THF/Pyridine/Water ( 8:1:1) (~1mL) and TEA/H2O/THF (8:1:1) (~1mL) for 3 minutes
Step 4: Flush column with acetonitrile and blow dry support
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