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Oligo Synthesis

Oligo Synthesis : CEPs

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dG-Thiophosphoramidite

dG-Thiophosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1720-02dG-Thiophosphoramidite 0.25g £336.00£319.20Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1720-90dG-Thiophosphoramidite 100µmoles £140.00£133.00Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

dG-Thiophosphoramidite

dG-Thiophosphoramidite

Glen Research

dG-Thiophosphoramidite

Structure

Catalog Number: 10-1720-xx

Description: dG-Thiophosphoramidite

5'-Dimethoxytrityl-N-isobutyryl-2'-deoxyGuanosine,
3'-[(Β-thiobenzoylethyl)-(1-pyrrolidinyl)]-thiophosphoramidite
Formula: C48H53N6O8PS2 M.W.: 937.07 F.W.: 361.34(dithioate)

Diluent: 10% (v/v) Anhydrous Dichloromethane in Anhydrous Acetonitrile
Coupling: See Technical Bulletin
Deprotection: See Technical Bulletin for details (Technical Bulletin).
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: No data available

THIOPHOSPHORAMIDITES

Replacing two non-bridging oxygen atoms with sulfur atoms in a DNA phosphodiester linkage creates a phosphorodithioate (PS2) linkage.1 Like natural DNA, the phosphorodithioate linkage is achiral at phosphorus. This analog is completely resistant to nuclease degradation and forms complexes with DNA and RNA with somewhat reduced stabilities.2 Moreover, it has been found that PS2-ODNs bind proteins with a higher affinity than their phosphodiester analogues2-6 suggesting that PS2-ODNs may have additional utility in the form of sulfur-modified phosphate ester aptamers (thioaptamers)3,6-8 for therapeutic and diagnostic applications. Thiophosphoramidites are now commercially available after recent work at AM Biotechnologies (www.thioaptamer.com).

  1. Thiophosphoramidites (ThioPAs) are not soluble in anhydrous acetonitrile diluent. Rather, 10% DCM (v/v) in acetonitrile is an ideal diluent for all four of the thioPAs for a final amidite concentration of 0.15 M.
  2. ThioPAs are somewhat less stable than normal DNA phosphoramidites in anhydrous acetonitrile containing 10% DCM; however, the coupling efficiency of all four thioPAs is not reduced after two days in solution at room temperature.
  3. After synthesis, the thioPA bottle on the synthesizer should be replaced with one containing acetonitrile diluent and the synthesizer line flushed with acetonitrile.

A typical cycle for the solid-phase synthesis of a PS2 linkage is different from a standard cycle for the synthesis of normal phosphate linkages. After coupling, the resulting thiophosphite triester is then sulfurized with DDTT. Capping is carried out AFTER sulfurization.

Upon completion of the automated synthesis, deprotection is carried out using a concentrated ammonia:ethanol (3:1, v:v) mix containing 20 mM DTT at 55 °C for 15-16 h.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

dG-Thiophosphoramidite

dG-Thiophosphoramidite

Glen Research

Material Safety Data Sheet

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

dG-Thiophosphoramidite

dG-Thiophosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.15M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1720-02 0.25grams .25grams 1.78 46 27.6 17.25 12.55 9.2 2.3
10-1720-90 100µmoles .094grams .67 9 5.4 3.38 2.45 1.8 .45
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm  
Approximate Number of Additions
10-1720-02 0.25grams .25grams 2.65 .1 46.6 29.13 21.18 2.91  
10-1720-90 100µmoles .094grams 1 .1 13.6 8.5 6.18 .85  
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm    
Approximate Number of Additions
10-1720-02 0.25grams .25grams 2.65 .1 48.2 30.13 21.91    
10-1720-90 100µmoles .094grams 1 .1 15.2 9.5 6.91    

08/15/2012 | http://www.glenres.com/ProductFiles/

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200