Catalog Number: 10-1058-xx
Diluent: 10% THF in Anhydrous Acetonitrile
|Coupling: Dissolve this product in anhydrous acetonitrile:THF (9:1). A 6 minute coupling time is recommended.
|Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
|Storage: Refrigerated storage, maximum of 2-8°C, dry
|Stability in Solution: 2-3 days
A molecular beacon probe1 has its natural fluorescence quenched in solution unless it is hybridized to the target sequence. Consequently, the design of a molecular beacon requires a fluorophore to be in one part of the sequence and the quencher molecule to be in another, with both molecules being separated from the oligonucleotide by a hydrocarbon spacer. The Dabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus. 5’-Dabcyl Phosphoramidite locates the quencher at the 5’-terminus and Dabcyl-dT places it within the sequence, leaving the 3’-terminus available for polymerase extension.
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