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Oligo Synthesis

Oligo Synthesis : CEPs

Prices quoted are for single packs only. For multiples of the same product please request a quote. Some of Glen's products are hazardous and may be subject to additional shipping charges. Full product information is available on Glen Research's website.

CleanAmp™-Ac-dC-CE Phosphoramidite

CleanAmp™-Ac-dC-CE Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1450-02CleanAmp™-Ac-dC-CE Phosphoramidite 0.25g £192.00£176.64Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1450-05CleanAmp™-Ac-dC-CE Phosphoramidite 0.5g £384.00£353.28Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order
10-1450-90CleanAmp™-Ac-dC-CE Phosphoramidite 100µmoles £80.00£73.60Offer until : 29-Feb-2020Offer Code : GLEN88% off all Glen Research products View Offer Quantity Add to Order

CleanAmp™-Ac-dC-CE Phosphoramidite

CleanAmp™-Ac-dC-CE Phosphoramidite

Glen Research

CleanAmp™-Ac-dC-CE Phosphoramidite

Structure

Catalog Number: 10-1450-xx

Description: CleanAmp™-Ac-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,
3'-[(4-oxo-tetradecyl)-(N,N-diisopropyl)]-phosphoramidite
Formula: C52H73N4O9P M.W.: 929.13 F.W.: FW: 499.54 (triester)

Diluent: Anhydrous Acetonitrile
Coupling: 10 minute coupling time. CleanAmp™ monomers must be used with UltraMild phosphoramidites and Cap Mix.
Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Glen Report 21.2 for details. (www.glenresearch.com/GlenReports/GR21-2.pdf)
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 24 hours

CleanAmp™ Primers

CleanAmp™ Primers offer an alternative to other Hot Start technologies and allow greater control of primer hybridization and extension during PCR. It has been demonstrated that CleanAmp™ Primers outperform other technologies in multiple applications. Indeed, over a broad range of applications, CleanAmp™ Primers reduce or eliminate off-target amplification. Greater amplicon yield is also achieved, due to improvement in specificity and sensitivity. By using either the slow-releasing Precision primers with two CleanAmp™ phosphotriester linkages or the faster-releasing Turbo Primers with a single CleanAmp™ phosphotriester linkage, the rate of formation of unmodified primer can be controlled to suit reaction needs. A table to aid in the selection of Turbo and Precision Primers for specific applications is shown below.

Turbo Primers Precision Primers
Fast cycling Standard cycling
Multiplex PCR One-step reverse-transcription PCR
Improves amplicon yield Improved specificity and limit of detection
Reduces mis-priming/ primer dimer formation Greatest reduction in mis-priming/primer dimer formation

Synthesis of CleanAmp™ Primers requires the use of UltraMild Chemistry.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

CleanAmp™-Ac-dC-CE Phosphoramidite

CleanAmp™-Ac-dC-CE Phosphoramidite

Glen Research

CleanAmp™-Ac-dC-CE Phosphoramidite

CleanAmp™-Ac-dC-CE Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1450-90 100µmoles .093grams 1 20 12 7.5 5.45 4 1
10-1450-02 0.25grams .25grams 2.69 76.33 45.8 28.63 20.82 15.27 3.82
10-1450-05 0.5grams .5grams 5.38 166 99.6 62.25 45.27 33.2 8.3
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1450-90 100µmoles .093grams 1.5 .07 23.6 14.75 10.73 1.48
10-1450-02 0.25grams .25grams 4.02 .07 74 46.25 33.64 4.63
10-1450-05 0.5grams .5grams 8.03 .07 154.2 96.38 70.09 9.64
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1450-90 100µmoles .093grams 1.5 .07 25.2 15.75 11.45

10-1450-02 0.25grams .25grams 4.02 .07 75.6 47.25 34.36

10-1450-05 0.5grams .5grams 8.03 .07 155.8 97.38 70.82

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200