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Oligo Synthesis

Oligo Synthesis : CEPs

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Cholesteryl-TEG Phosphoramidite

Cholesteryl-TEG Phosphoramidite

Glen Research

Catalogue No.DescriptionPack SizePriceQty
10-1975-02Cholesteryl-TEG Phosphoramidite 0.25g £436.00£414.20Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1975-90Cholesteryl-TEG Phosphoramidite 100µmoles £212.00£201.40Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order
10-1975-95Cholesteryl-TEG Phosphoramidite 50µmoles £112.00£106.40Offer until : 31-Dec-2020Offer Code : GLEN55% off all Glen products View Offer Quantity Add to Order

Cholesteryl-TEG Phosphoramidite

Cholesteryl-TEG Phosphoramidite

Glen Research

Cholesteryl-TEG Phosphoramidite

Structure

Catalog Number: 10-1975-xx

Description: Cholesteryl-TEG Phosphoramidite

1-Dimethoxytrityloxy-3-O-(N-cholesteryl-3-aminopropyl)-triethyleneglycol-
glyceryl-2-O-(2-cyanoethyl)-(N,N,-diisopropyl)-phosphoramidite
Formula: C70H106N3O11P M.W.: 1196.60 F.W.: 755.97

Diluent: 10% THF in Anhydrous Acetonitrile
Add fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf.
Coupling: 15 minute coupling time. To maintain label yield, carry out the synthesis DMT-on.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. To maintain label yield, remove the 5'-DMT after ammonium hydroxide deprotection.
Storage: Freezer storage, -10 to -30°C, dry
Stability in Solution: 2-3 days

CHOLESTEROL LABELLING

Potential therapeutic oligonucleotides must permeate the cell membrane for optimal activity. The addition of lipophilic groups to an oligonucleotide would be expected to enhance activity. The use of cholesteryl oligos and the consequent improvement in activity has been described. We have designed our Cholesteryl Phosphoramidite with our branched triethyleneglycol (TEG) spacer for maximum solubility in acetonitrile as well as for applications requiring multiple labels.

 

 

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Cholesteryl-TEG Phosphoramidite

Cholesteryl-TEG Phosphoramidite

Glen Research

Cholesteryl-TEG Phosphoramidite

Cholesteryl-TEG Phosphoramidite

Glen Research

DILUTION/COUPLING DATA

The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.

ABI 392/394
Cat.No. Pack
Size
Grams/
Pack
0.1M Dil.
(mL)
LV40 LV200 40nm 0.2µm 1µm 10µm
Approximate Number of Additions
10-1975-02 0.25grams .25grams 2.09 56.33 33.8 21.13 15.36 11.27 2.82
10-1975-90 100µmoles .12grams 1 20 12 7.5 5.45 4 1
10-1975-95 50µmoles .06grams .5 3.33 2 1.25 .91 .67 .17
Expedite
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 50nm 0.2µm 1µm 15µm
Approximate Number of Additions
10-1975-02 0.25grams .25grams 3.12 .07 56 35 25.45 3.5
10-1975-90 100µmoles .12grams 1.5 .07 23.6 14.75 10.73 1.48
10-1975-95 50µmoles .06grams .75 .07 8.6 5.38 3.91 .54
Beckman
Cat.No. Pack
Size
Grams/
Pack
Dilution
(mL)
Molarity 30nm 200nm 1000nm

Approximate Number of Additions
10-1975-02 0.25grams .25grams 3.12 .07 57.6 36 26.18

10-1975-90 100µmoles .12grams 1.5 .07 25.2 15.75 11.45

10-1975-95 50µmoles .06grams .75 .07 10.2 6.38 4.64

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200